Hail Daymon, Lauzìere Isabelle, Dowd Scot E, Bextine Blake
University of Texas at Tyler, 3900 University Blvd., Tyler, TX 75799, USA.
Environ Entomol. 2011 Feb;40(1):23-9. doi: 10.1603/EN10115.
The glassy-winged sharpshooter, Homalodisca vitripennis (Germar), is an invasive pest that has spread across the southern and western United States. H. vitripennis is highly polyphagous and voracious, feeding on at least 100 plant species and consuming up to 100 times its weight in xylem fluid daily. The insect is a vector of the phytopathogen Xylella fastidiosa (Wells), which is the causative agent of Pierce's disease in grapevines. To evaluate the microbial flora associated with H. vitripennis, total DNA extracts from hemolymph, alimentary canal excretions, and whole insect bodies were subjected to 16S rDNA pyrosequencing using the bTEFAP methodology and the resulting sequences (370-520 bp in length) were compared with a curated high quality 16S database derived from GenBank http://www.ncbi.nlm.nih.gov. Species from the genera Wolbachia, Delftia (formerly Pseudomonas), Pectobacterium, Moraxella, Serratia, Bacillus, and many others were detected and a comprehensive picture of the microbiome associated with H. vitripennis was established. Some of the bacteria identified in this report are initial discoveries; providing a breadth of knowledge to the microbial flora of this insect pest can serve as a reservoir of information for developing biological control strategies.
玻璃翅叶蝉（Homalodisca vitripennis (Germar)）是一种入侵性害虫，已在美国南部和西部蔓延。玻璃翅叶蝉食性高度杂且食量很大，以至少100种植物为食，每天消耗的木质部汁液重量可达其自身重量的100倍。这种昆虫是植物病原体木质部难养菌（Xylella fastidiosa (Wells)）的传播媒介，该病原体是葡萄藤上皮尔氏病的致病因子。为了评估与玻璃翅叶蝉相关的微生物群落，使用bTEFAP方法对血淋巴、消化道排泄物和整个昆虫体的总DNA提取物进行16S rDNA焦磷酸测序，并将所得序列（长度为370 - 520 bp）与来自GenBank（http://www.ncbi.nlm.nih.gov）的经过整理的高质量16S数据库进行比较。检测到了沃尔巴克氏体属、代尔夫特菌属（以前的假单胞菌属）、果胶杆菌属、莫拉克斯氏菌属、沙雷氏菌属、芽孢杆菌属等许多属的物种，并建立了与玻璃翅叶蝉相关的微生物群落的全面图谱。本报告中鉴定出的一些细菌是首次发现；对这种害虫的微生物群落有更广泛的了解可为制定生物防治策略提供丰富的信息储备。
