PTGDR-441C/T 多态性与北印度人群哮喘无关。
No association of PTGDR -441C/T polymorphism with asthma in a North Indian population.
机构信息
Department of Biotechnology, Panjab University, Chandigarh, India.
出版信息
Dis Markers. 2011;31(6):353-9. doi: 10.3233/DMA-2011-0850.
BACKGROUND
Asthma is the most prevalent disease in India according to the national survey conducted by NFHS 2 in 1998-399. Prostaglandin D2 (PGD2) is a bronchoconstriction inducing metabolite of arachidonic acid in the mast cells, which is produced on exposure to allergens and acts as a ligand for the Prostaglandin D2 Receptor (PTGDR). Polymorphisms in the PTGDR gene have been suggested to be involved in the mechanism of asthma.
OBJECTIVE
This is the first study conducted in India, investigating the role of PTGDR -441C/T} promoter polymorphism in asthma pathogenesis.
METHODS
A case-control study was performed with a total of 992 subjects, including 410 adult asthmatics and 582 healthy controls from regions of North India. The PTGDR -441C/T polymorphism was genotyped by Tetra-Primer Amplification Refractory Mutation System Polymerase Chain Reaction (Tetra-Primer ARMS PCR).
RESULTS
Statistical analysis of the results between asthma cases and controls for the PTGDR −441C/T polymorphism showed Chi² (χ²) = 0.29, OR = 0.95, 95% CI (0.70-1.15) and p = 0.599. Neither the genotypic nor the allelic frequencies observed for the PTGDR −441C/T polymorphism, were significantly associated with asthma or asthma phenotypes.
CONCLUSIONS
The PTGDR -441C/T polymorphism is not associated with asthma or its phenotypes in the studied North Indian population.
背景
根据 1998-399 年国家家庭健康调查进行的全国调查，哮喘是印度最常见的疾病。前列腺素 D2（PGD2）是肥大细胞中花生四烯酸的支气管收缩诱导代谢物，在暴露于过敏原时产生，并作为前列腺素 D2 受体（PTGDR）的配体。PTGDR 基因中的多态性被认为与哮喘的发病机制有关。
目的
这是在印度进行的第一项研究，旨在调查 PTGDR-441C/T 启动子多态性在哮喘发病机制中的作用。
方法
进行了一项病例对照研究，共有 992 名受试者，包括来自印度北部地区的 410 名成年哮喘患者和 582 名健康对照者。PTGDR-441C/T 多态性通过四引物扩增受阻突变系统聚合酶链反应（Tetra-Primer ARMS PCR）进行基因分型。
结果
对哮喘病例和对照组之间的 PTGDR-441C/T 多态性进行统计分析，结果显示 Chi²（χ²）= 0.29，OR = 0.95，95%CI（0.70-1.15），p = 0.599。PTGDR-441C/T 多态性的基因型和等位基因频率与哮喘或哮喘表型均无显著相关性。
结论
在研究的印度北部人群中，PTGDR-441C/T 多态性与哮喘或其表型无关。
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