Department of Nutrigenomics, National Food and Nutrition Institute, Warsaw, Poland.
Mol Biol Rep. 2012 May;39(5):5699-704. doi: 10.1007/s11033-011-1378-x. Epub 2011 Dec 20.
Endogenous DNA damage levels were analyzed in relation to polymorphisms in genes encoding phase I detoxifying enzyme-CYP1A1, phase II detoxifying enzymes-GSTM1, GSTT1, GSTP1 and enzyme involved in nucleotide excision repair-XPD. The study group consisted of 220 healthy non-smoking volunteers; 90 men and 130 woman, 25-60 years old (44 ± 10 years). The level of DNA damage (% DNA in tail) was evaluated by alkaline comet assay. The genetic variants were determined by restriction fragment length polymorphism PCR. The highest level of DNA damage (6.7%) was found in carriers of both: AA variant of XPD gene and M1 null variant of GSTM1 gene. The lowest level of DNA breaks (3.7%) was associated with the genotype GSTP1-AA/GSTM1 (+).
对与细胞色素 P4501A1（CYP1A1）、谷胱甘肽 S-转移酶 M1（GSTM1）、谷胱甘肽 S-转移酶 T1（GSTT1）、谷胱甘肽 S-转移酶 P1（GSTP1）和核苷酸切除修复酶 XPD 基因多态性相关的内源性 DNA 损伤水平进行了分析。研究组包括 220 名健康不吸烟志愿者；90 名男性和 130 名女性，年龄 25-60 岁（44±10 岁）。通过碱性彗星试验评估 DNA 损伤水平（%DNA 在尾部）。通过限制性片段长度多态性 PCR 确定遗传变异。在 XPD 基因 AA 变异和 GSTM1 基因 M1 缺失变异的携带者中发现最高水平的 DNA 损伤（6.7%）。与 GSTP1-AA/GSTM1（+）基因型相关的 DNA 断裂最低水平（3.7%）。
