Tsui Kyle, Durbic Tanja, Gebbia Marinella, Nislow Corey
The Donnelly Centre for Biomolecular Research, University of Toronto, Toronto, ON, Canada.
Methods Mol Biol. 2012;833:389-411. doi: 10.1007/978-1-61779-477-3_23.
The basic unit of chromatin is double-stranded DNA wrapped around nucleosome core particles, the -classic "beads-on-a-string" described by Kornberg and colleagues. The history of chromatin studies has experienced many peaks, from the earliest studies by Miescher to the biochemical studies of the 1960s and 1970s, the appreciation for the influence of histone modifications in controlling gene expression in the 1990s to the genome-wide studies that began in 2006 and show no signs of abating with the introduction of next generation sequencing technologies. Genome-wide studies not only have provided a base line to understand relationships between chromatin structure and gene function but also have begun to provide new insights into chromatin remodelling. Here, we describe the use of genome-wide approaches to determining nucleosome occupancy in yeast.
染色质的基本单位是缠绕在核小体核心颗粒周围的双链DNA，即科恩伯格及其同事所描述的经典的“串珠”结构。染色质研究的历史经历了许多高峰，从最早由米舍尔进行的研究，到20世纪60年代和70年代的生化研究，再到20世纪90年代对组蛋白修饰在控制基因表达方面影响的认识，直至2006年开始的全基因组研究，而且随着新一代测序技术的引入，这些研究毫无减弱的迹象。全基因组研究不仅为理解染色质结构与基因功能之间的关系提供了基线，还开始为染色质重塑提供新的见解。在此，我们描述了使用全基因组方法来确定酵母中的核小体占据情况。
