针对植物真菌病原体的杀真菌剂抗性测定

Fungicide resistance assays for fungal plant pathogens.

作者信息

Secor Gary A, Rivera Viviana V

机构信息

Department of Plant Pathology, North Dakota State University, Fargo, ND, USA.

出版信息

Methods Mol Biol. 2012;835:385-92. doi: 10.1007/978-1-61779-501-5_23.

DOI:10.1007/978-1-61779-501-5_23

PMID:22183666

Abstract

Fungicide resistance assays are useful to determine if a fungal pathogen has developed resistance to a fungicide used to manage the disease it causes. Laboratory assays are used to determine loss of sensitivity, or resistance, to a fungicide and can explain fungicide failures and for developing successful fungicide recommendations in the field. Laboratory assays for fungicide resistance are conducted by measuring reductions in growth or spore germination of fungi in the presence of fungicide, or by molecular procedures. This chapter describes two techniques for measuring fungicide resistance, using the sugarbeet leaf spot fungus Cercospora beticola as a model for the protocol. Two procedures are described for fungicides from two different classes; growth reduction for triazole (sterol demethylation inhibitor; DMI) fungicides, and inhibition of spore germination for quinone outside inhibitor (QoI) fungicides.

摘要

杀菌剂抗性测定对于确定真菌病原体是否对用于防治其所致病害的杀菌剂产生抗性很有用。实验室测定用于确定对杀菌剂的敏感性丧失或抗性，可解释杀菌剂失效情况，并为在田间制定成功的杀菌剂使用建议提供依据。杀菌剂抗性的实验室测定通过测量在杀菌剂存在下真菌生长或孢子萌发的减少情况，或通过分子程序来进行。本章以甜菜叶斑病菌尾孢菌为该方案的模型，描述了两种测定杀菌剂抗性的技术。针对两类不同的杀菌剂描述了两种方法；三唑类（甾醇脱甲基抑制剂；DMI）杀菌剂的生长抑制测定方法，以及醌外抑制剂（QoI）杀菌剂的孢子萌发抑制测定方法。