Liu Pengfei, Wang Haiqiang, Zhou Yuxin, Meng Qingxiao, Si Naiguo, Hao Jianjun J, Liu Xili
Department of Plant Pathology, China Agricultural University, Beijing, China.
State Key Laboratory of the Discovery and Development of Novel Pesticide, China Shenyang Research Institute of the Chemical Industry, Shenyang, China.
Pestic Biochem Physiol. 2014 Jun;112:19-25. doi: 10.1016/j.pestbp.2014.05.004. Epub 2014 Jun 4.
Enestroburin and SYP1620 are newly developed strobilurin chemicals carrying fungicidal activity and need to be fully characterized in activities of anti-oomycete or anti-fungi, disease prevention and systemic translocation in planta. Their inhibitory activities were examined by amending the chemical in agar media, on which selected plant pathogens were grown and mycelial growth were measured. Effective concentrations for 50% inhibition (EC50) of mycelial growth were calculated to determine the level of fungicide sensitivity of the pathogen. Azoxystrobin was used as control. To examine the prevention and systemic translocation in plants, the fungicides were either sprayed on wheat leaves or dipped on wheat roots, which then were detected using high performance liquid chromatography. All the three fungicides inhibited mycelial growth of Sphacelotheca reiliana, Phytophthora infestans, Peronophythora litchi, and Magnaporthe oryzae, with EC50 values ranging from 0.02 to 2.84μg/ml; EC50 of SYP1620 was significantly lower than that of azoxystrobin and enestroburin on Valsa mali, Gaeumannomyces graminis, Alternaria solani, and Colletotrichun orbiculare. The three QoI fungicides showed strong inhibitory activities on spore germination against the 13 pathogens tested and were highly effective on biotrophic pathogens tested. Enestroburin and SYP1620 penetrated and spread in wheat leaves, but the penetration and translocation levels were lower compared to azoxystrobin. The three fungicides were all rapidly taken up by wheat roots and transported upwards, with greater fungicide concentrations in roots than in stems and leaves. The results indicate that enestroburin and SYP1620 are systemic fungicides that inhibit a broad spectrum of fungi and oomycetes.
烯肟菌酯和SYP1620是新开发的具有杀菌活性的甲氧基丙烯酸酯类化合物，需要对其抗卵菌或抗真菌活性、防病活性及在植物体内的系统传导性进行全面表征。通过在琼脂培养基中添加这些化合物来检测其抑制活性，在该培养基上培养选定的植物病原体并测量菌丝生长。计算菌丝生长50%抑制率（EC50）的有效浓度，以确定病原体对杀菌剂的敏感程度。嘧菌酯用作对照。为了检测这些杀菌剂在植物中的防病和系统传导性，将杀菌剂喷洒在小麦叶片上或浸蘸在小麦根部，然后使用高效液相色谱法进行检测。所有这三种杀菌剂均抑制玉米丝黑穗病菌、致病疫霉、荔枝霜霉病菌和稻瘟病菌的菌丝生长，EC50值在0.02至2.84μg/ml之间；在苹果腐烂病菌、禾顶囊壳、番茄早疫病菌和西瓜炭疽病菌上，SYP1620的EC50显著低于嘧菌酯和烯肟菌酯。这三种甲氧基丙烯酸酯类杀菌剂对所测试的13种病原体的孢子萌发均表现出较强的抑制活性，对所测试的活体营养型病原体高效。烯肟菌酯和SYP1620能在小麦叶片中渗透和扩散，但与嘧菌酯相比，其渗透和传导水平较低。这三种杀菌剂均能被小麦根部迅速吸收并向上运输，根部的杀菌剂浓度高于茎部和叶片。结果表明，烯肟菌酯和SYP1620是能抑制多种真菌和卵菌的内吸性杀菌剂。
