Nadal Marina, Gold Scott E
Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland.
Methods Mol Biol. 2012;835:481-9. doi: 10.1007/978-1-61779-501-5_29.
Autophagy is a complex degradative process by which cytosolic material, including organelles, is randomly sequestered within double-membrane bound vesicles termed autophagosomes and targeted for degradation. Initially described as a nutrient stress adaptation response, the process of autophagy is now recognized as a central mechanism involved in many developmental processes. In this chapter, we provide guidelines to assess the initial steps of autophagy by monitoring autophagic body vacuolar accumulation. We employed a standard electron microscopy approach to observe the vacuoles of nutrient stressed fungal cells.
自噬是一个复杂的降解过程，通过该过程，包括细胞器在内的胞质物质被随机隔离在称为自噬体的双膜结合囊泡中，并被靶向降解。自噬最初被描述为一种营养应激适应反应，现在被认为是许多发育过程中的核心机制。在本章中，我们提供了通过监测自噬体空泡积累来评估自噬初始步骤的指南。我们采用标准电子显微镜方法观察营养应激真菌细胞的空泡。
