利用DNA宏阵列检测植物和土壤样本中的真菌植物病原体。
Fungal plant pathogen detection in plant and soil samples using DNA macroarrays.
作者信息
Lievens B, Justé A, Willems K A
机构信息
Laboratory for Process Microbial Ecology and Bioinspirational Management, Consortium for Industrial Microbiology and Biotechnology, Department of Microbial and Molecular Systems, K.U. Leuven Association, Lessius Mechelen, Campus De Nayer, Sint-Katelijne-Waver, Belgium.
出版信息
Methods Mol Biol. 2012;835:491-507. doi: 10.1007/978-1-61779-501-5_30.
PCR-based DNA array technology is one of the most suitable techniques to detect and identify multiple pathogens in a single assay. Out of the different array platforms that currently exist, membrane-based DNA macroarrays are the most convenient for plant disease diagnosis because of low costs, great sensitivity, and modest equipment requirements. Here we describe a protocol for routine detection of plant pathogens using DNA macroarrays, i.e., from sampling to analysis of hybridization results. Diagnosis can be completed within 36 h after sample collection.
基于聚合酶链反应（PCR）的DNA芯片技术是在一次检测中同时检测和鉴定多种病原体的最合适技术之一。在目前存在的不同芯片平台中，基于膜的DNA宏阵列因其成本低、灵敏度高和设备要求适中而最便于用于植物病害诊断。在此，我们描述一种使用DNA宏阵列常规检测植物病原体的方法，即从采样到杂交结果分析。样本采集后36小时内即可完成诊断。
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