聚羟基丁酸酯的表达作为大豆种皮代谢工程的模型。
Expression of polyhydroxybutyric acid as a model for metabolic engineering of soybean seed coats.
机构信息
Ottawa-Carleton Institute of Biology, University of Ottawa, PO Box 450, Station A, Ottawa, ON K1N 6N5, Canada.
出版信息
Transgenic Res. 2012 Aug;21(4):895-9. doi: 10.1007/s11248-011-9575-x. Epub 2011 Dec 20.
The feasibility of genetically engineering soybean seed coats to divert metabolism towards the production of novel biochemicals was tested. The genes phbA, phbB, phbC from Ralstonia eutropha each under the control of the seed coat peroxidase promoter were introduced into soybean and the production of polyhydroxybutyrate (PHB) was assayed. The analysis of seed coats arising from 4 independent transformation events demonstrated that PHB was produced at a mean of 0.12% seed coat dried weight with individual values up to 0.36%. These values demonstrate that it is possible to metabolically engineer soybean seed coats.
研究了通过基因工程改变大豆种皮的代谢途径来生产新型生化物质的可行性。将 Ralstonia eutropha 的 phbA、phbB 和 phbC 基因分别置于种子 coat 过氧化物酶启动子的控制下导入大豆，并检测聚羟基丁酸酯（PHB）的产量。对来自 4 个独立转化事件的种皮分析表明，PHB 的产量平均为种皮干重的 0.12%，个别值高达 0.36%。这些值表明，大豆种皮的代谢工程是可行的。
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