Kanizay Lisa, Jacobs Thomas, Hancock C Nathan
Center for Applied Genetic Technologies, University of Georgia, 111 Riverbend Rd, Athens, GA, 30602, USA.
Institute for Plant Breeding, Genetics and Genomics, University of Georgia, 111 Riverbend Rd, Athens, GA, 30602, USA.
Transgenic Res. 2016 Apr;25(2):187-93. doi: 10.1007/s11248-015-9922-4. Epub 2015 Dec 10.
Most soybean cultivars produce buff colored seeds due to a seed coat specific siRNA mechanism. This phenomenon is specifically limited to the seed coat and produces a strong visual effect, thus, a strategy to evade the silencing was used to produce a maternal transgenic marker for soybeans. Expression of a rice chalcone synthase transgene with little DNA sequence homology to the soybean siRNAs resulted in dark colored seed coats. This phenotype is the result of anthocyanin pigment production and does not appear to affect other tissues. This novel approach for producing an easily scored transgenic marker for soybean will facilitate high-throughput screening and analysis of transgenic soybean.
由于种皮特异性小干扰RNA机制，大多数大豆品种产生浅黄色种子。这种现象特别局限于种皮，并产生强烈的视觉效果，因此，采用了一种规避沉默的策略来生产大豆母体转基因标记。与大豆小干扰RNA几乎没有DNA序列同源性的水稻查尔酮合酶转基因的表达导致种皮颜色变深。这种表型是花青素色素产生的结果，似乎不影响其他组织。这种为大豆生产易于评分的转基因标记的新方法将有助于高通量筛选和分析转基因大豆。
