Key Laboratory of Engineering Research of Veterinary Bio-products of Agricultural Ministry, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, National Center for Engineering Research of Veterinary Bio-products, Zhongling St 50#, Nanjing 210014, China.
Vet Rec. 2012 Feb;170(7):178. doi: 10.1136/vr.100325. Epub 2011 Dec 20.
Ruminants are an important reservoir of Escherichia coli O157:H7. To reduce E coli O157:H7 excretion by these animals could play a key role in prevention and control of human infections. In the present study, the authors used 12 three-month-old goats to evaluate the efficacy of intranasal administration of the Stx2B-Tir-Stx1B-Zot protein. These goats were inoculated on days 0 and 21 and infected with 10(10) colony-forming units (cfu) of E coli O157:H7 by oral inoculation on day 36. Faecal shedding was monitored daily for two weeks. All of six goats immunised with recombinant protein elicited significant Stx2b-Tir-Stx1b-Zot-specific serum IgG antibodies, and three of them also showed production of antigen-specific IgA in faeces. The immunised goats showed much less shedding of E coli O157:H7 after challenge. These results demonstrate the potential for the use of Stx2B-Tir-Stx1B-Zot protein in mucosal vaccine formulations to prevent colonisation and shedding of E coli O157:H7 in goats.
反刍动物是大肠杆菌 O157:H7 的重要储存库。减少这些动物的大肠杆菌 O157:H7 排泄可能在预防和控制人类感染方面发挥关键作用。在本研究中，作者使用 12 只三个月大的山羊来评估 Stx2B-Tir-Stx1B-Zot 蛋白鼻内给药的疗效。这些山羊在第 0 天和第 21 天接种，并在第 36 天通过口服接种 10(10)个菌落形成单位（cfu）的大肠杆菌 O157:H7 进行感染。每天监测粪便脱落情况两周。用重组蛋白免疫的 6 只山羊均产生了显著的 Stx2b-Tir-Stx1b-Zot 特异性血清 IgG 抗体，其中 3 只还在粪便中产生了抗原特异性 IgA。免疫山羊在挑战后大肠杆菌 O157:H7 的脱落明显减少。这些结果表明，Stx2B-Tir-Stx1B-Zot 蛋白有可能用于粘膜疫苗制剂，以防止山羊中大肠杆菌 O157:H7 的定植和脱落。
