细胞和血管侵犯 Integra 和 Strattice 的数学建模和频率梯度分析：走向组织再生支架的最佳设计。
Mathematical modeling and frequency gradient analysis of cellular and vascular invasion into integra and strattice: toward optimal design of tissue regeneration scaffolds.
机构信息
New York and Ithaca, N.Y. From the Division of Plastic Surgery, Weill Cornell Medical College, and the School of Chemical and Biomolecular Engineering, the Department of Biomedical Engineering, and the Sibley School of Mechanical and Aerospace Engineering, Cornell University.
出版信息
Plast Reconstr Surg. 2012 Jan;129(1):89-99. doi: 10.1097/PRS.0b013e318230c5f1.
BACKGROUND
Rapid, effective host cell invasion and vascularization is essential for durable incorporation of avascular tissue-replacement scaffolds. In this study, the authors sought to qualitatively and quantitatively determine which of two commercially available products (i.e., Strattice and Integra) facilitates more rapid cellular and vascular invasion in a murine model of graft incorporation.
METHODS
Integra and Strattice were implanted subcutaneously into the dorsa of C57BL/6 mice; harvested after 3, 7, or 14 days; and stained with hematoxylin and eosin, 4',6-diamidino-2-phenylindole, and immunohistochemical stains for CD31 and α-smooth muscle actin. Exponential decay equations describing cellular invasion through each layer were fit to each material/time point. Mean cell density and cell frequency maps were created denoting extent of invasion by location within the scaffold.
RESULTS
Qualitative analysis demonstrated extensive cellular infiltration into Integra by 3 days and increasing over the remaining 14 days. Invasion of Strattice was sparse, even after 14 days. α-Smooth muscle actin immunohistochemistry revealed blood vessel formation within Integra by 14 days but no analogous neovascularization in Strattice. Mean decay equations for Integra and Strattice were y = 76.3(0.59) and y = 75.5(0.33), respectively. Both cell density measurements and frequency mapping demonstrated that, at all time points, Integra manifested a greater density/depth of cellular invasion when compared with Strattice.
CONCLUSIONS
These data confirm empiric clinical observations that Integra is more rapidly invaded than Strattice when placed in a suitable host bed. A remnant microvasculature template is not sufficient for effective cellular ingrowth into an artificial tissue construct. These findings provide insight into means for improving future dermal replacement products.
背景
快速有效的宿主细胞入侵和血管生成对于无血管组织替代支架的持久植入至关重要。在这项研究中，作者试图定性和定量地确定两种市售产品（即 Strattice 和 Integra）中哪一种更能促进植入物中移植物更快地细胞和血管侵入。
方法
Integra 和 Strattice 被植入 C57BL/6 小鼠的背部皮下；在 3、7 或 14 天后收获；并用苏木精和伊红、4'，6-二脒基-2-苯基吲哚和 CD31 和α-平滑肌肌动蛋白的免疫组织化学染色染色。描述每个材料/时间点细胞入侵的指数衰减方程拟合到每个材料/时间点。通过在支架内的位置创建平均细胞密度和细胞频率图来创建入侵程度的表示。
结果
定性分析表明，Integra 在 3 天内大量细胞浸润，并在接下来的 14 天内逐渐增加。Strattice 的入侵则稀疏，即使在 14 天后也是如此。α-平滑肌肌动蛋白免疫组织化学显示，Integra 在 14 天内形成血管，但在 Strattice 中没有类似的新血管生成。Integra 和 Strattice 的平均衰减方程分别为 y = 76.3(0.59)和 y = 75.5(0.33)。细胞密度测量和频率映射均表明，在所有时间点，Integra 比 Strattice 表现出更大的细胞入侵密度/深度。
结论
这些数据证实了经验性临床观察，即在适当的宿主床中放置时，Integra 比 Strattice 更快地被入侵。残留的微血管模板不足以有效细胞进入人工组织构建物。这些发现为改善未来的皮肤替代产品提供了深入的见解。
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