LMDF-SME, Laboratoire de Microbiologie du Froid-Signaux et Micro-Environnement, EA 4312, Université de Rouen, Rouen, France.
J Appl Microbiol. 2012 Mar;112(3):502-11. doi: 10.1111/j.1365-2672.2011.05224.x. Epub 2012 Jan 24.
Pulsed light (PL) technology is an efficient surface decontamination process. Used in low transmitted energy conditions, PL induces a stress that can be perceived by bacteria. The effect of such a PL stress was investigated on the highly environmental adaptable germ Pseudomonas aeruginosa PAO1.
Pulses of transmitted energy (fluence) reaching 1·8Jcm(-2) can kill 10(9) bacteria. Application of a lower sublethal PL dose allowed the bacteria to resist and survive more efficiently to a subsequent dose of PL. This sublethal dose was not increasing the mutation frequency of Ps. aeruginosa, but altered the abundance of 15 proteins as revealed by a global proteome analysis, including stress-induced proteins, phage-related proteins, energy and carbon metabolisms, cell motility, and transcription and translation regulators.
A response to a low-energy PL dose takes place in Ps. aeruginosa, reducing the energy conversion systems, while increasing transcription and translation processes to produce proteins involved in chaperone mechanisms and phage-related proteins, probably to protect the bacterium against a new PL-induced stress.
Taken together, these results suggest that a low-energy PL dose is sufficient to provoke adaptation of Ps. aeruginosa, leading to enhancing its resistance to a subsequent lethal treatment.
脉冲光（PL）技术是一种有效的表面去污过程。在低传输能量条件下使用时，PL 会产生一种细菌能够感知到的应力。本研究旨在调查这种 PL 应力对高度适应环境的细菌铜绿假单胞菌 PAO1 的影响。
传输能量（辐照度）达到 1.8Jcm(-2) 的脉冲可以杀死 10(9)个细菌。应用较低的亚致死 PL 剂量可以使细菌更有效地抵抗和存活，以应对随后的 PL 剂量。这种亚致死剂量不会增加铜绿假单胞菌的突变频率，但通过全蛋白质组分析揭示了 15 种蛋白质的丰度发生了变化，包括应激诱导蛋白、噬菌体相关蛋白、能量和碳代谢、细胞运动以及转录和翻译调节剂。
铜绿假单胞菌对低能量 PL 剂量的反应会降低能量转换系统，同时增加转录和翻译过程，以产生参与伴侣机制和噬菌体相关蛋白的蛋白质，可能是为了保护细菌免受新的 PL 诱导的应激。
综上所述，这些结果表明，低能量 PL 剂量足以引起铜绿假单胞菌的适应，从而增强其对随后致死处理的抵抗力。
