神经节苷脂 GQ1b 在小鼠胚胎干细胞神经元细胞分化中的调控作用。

Regulatory roles of ganglioside GQ1b in neuronal cell differentiation of mouse embryonic stem cells.

机构信息

Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan 570-749 Korea.

出版信息

BMB Rep. 2011 Dec;44(12):799-804. doi: 10.5483/bmbrep.2011.44.12.799.

DOI:10.5483/bmbrep.2011.44.12.799

Abstract

Gangliosides play an important role in neuronal differentiation processes. The regulation of ganglioside levels is related to the induction of neuronal cell differentiation. In this study, the ST8Sia5 gene was transfected into mESCs and then differentiated into neuronal cells. Interestingly, ST8Sia5 gene transfected mESCs expressed GQ1b by HPTLC and immunofluorescence analysis. To investigate the effects of GQ1b over-expression in neurogenesis, neuronal cells were differentiated from GQ1b expressing mESCs in the presence of retinoic acid. In GQ1b expressing mESCs, increased EBs formation was observed. After 4 days, EBs were co-localized with GQ1b and nestin, and GFAP. Moreover, GQ1b co-localized with MAP-2 expressing cells in GQ1b expressing mESCs in 7-day-old EBs. Furthermore, GQ1b expressing mESCs increased the ERK1/2 MAP kinase pathway. These results suggest that the ST8Sia5 gene increases ganglioside GQ1b and improves neuronal differentiation via the ERK1/2 MAP kinase pathway.

摘要

神经节苷脂在神经元分化过程中发挥重要作用。神经节苷脂水平的调节与神经元细胞分化的诱导有关。在这项研究中，将 ST8Sia5 基因转染到 mESCs 中，然后分化为神经元细胞。有趣的是，ST8Sia5 基因转染的 mESCs 通过 HPTLC 和免疫荧光分析表达 GQ1b。为了研究 GQ1b 过表达对神经发生的影响，在存在维甲酸的情况下，从表达 GQ1b 的 mESCs 中分化神经元细胞。在表达 GQ1b 的 mESCs 中，观察到 EB 形成增加。4 天后，EBs 与 GQ1b 和巢蛋白、GFAP 共定位。此外，在 7 天龄的 EB 中，表达 GQ1b 的 mESCs 中 GQ1b 与表达 MAP-2 的细胞共定位。此外，表达 GQ1b 的 mESCs 增加了 ERK1/2 MAP 激酶通路。这些结果表明，ST8Sia5 基因通过 ERK1/2 MAP 激酶通路增加神经节苷脂 GQ1b，改善神经元分化。