Computational Genomics Department, Instituto Nacional de Medicina Genómica, Mexico City, Mexico.
J Nutr. 2012 Feb;142(2):278-83. doi: 10.3945/jn.111.152421. Epub 2011 Dec 21.
The R230C variant of the ATP-binding cassette transporter A1 (ABCA1) gene has been consistently associated with decreased HDL-cholesterol (HDL-C) concentrations in several studies in the Mexican mestizo population. However, information on how diet composition modifies the effect of the ABCA1-R230C variant on HDL-C concentrations is very scarce. The aim of the present study was to analyze whether the effect of ABCA1-R230C on HDL-C concentrations is modulated by dietary factors in a nationwide population sample of 3591 adults from the National Health and Nutrition Survey conducted by the State's Employees' Social Security and Social Services Institute. All participants answered a validated questionnaire to assess health status and weekly food consumption. Fasting blood samples were drawn for biochemical analysis and DNA extraction, and the ABCA1-R230C variant was genotyped using TaqMan assays. Statistical analyses consisted of simple linear and multiple regression modeling adjusting for age, BMI, smoking, and alcohol consumption. The overall C risk allele frequency was 9.3% and the variant was significantly associated with low HDL-C concentrations in both sexes. A significant negative correlation between carbohydrate consumption and HDL-C concentrations was observed in women bearing the R230C variant (P = 0.021) and a significant gene-diet interaction was found only in premenopausal women (P = 0.037). In conclusion, the effect of the ABCA1-R230C gene variant on HDL-C concentrations is modulated by carbohydrate intake in premenopausal women. This finding may help design optimized dietary interventions according to sex and ABCA1-R230C genotype.
ATP 结合盒转运蛋白 A1（ABCA1）基因的 R230C 变体与几种墨西哥梅斯蒂索人群的研究中 HDL-胆固醇（HDL-C）浓度降低有关。然而，关于饮食成分如何改变 ABCA1-R230C 变体对 HDL-C 浓度的影响的信息非常有限。本研究旨在分析 ABCA1-R230C 对 HDL-C 浓度的影响是否受全国范围内 3591 名成年人的饮食因素调节，这些成年人来自州雇员社会保障和社会服务研究所进行的国家健康和营养调查。所有参与者都回答了一份经过验证的问卷，以评估健康状况和每周的食物消费。抽取空腹血样进行生化分析和 DNA 提取，并使用 TaqMan 测定法对 ABCA1-R230C 变体进行基因分型。统计分析包括简单线性和多元回归建模，调整年龄、BMI、吸烟和饮酒。总体 C 风险等位基因频率为 9.3%，该变体与两性的低 HDL-C 浓度显著相关。在携带 R230C 变体的女性中，碳水化合物消耗与 HDL-C 浓度之间存在显著负相关（P=0.021），并且仅在绝经前女性中发现了显著的基因-饮食相互作用（P=0.037）。总之，ABCA1-R230C 基因变体对 HDL-C 浓度的影响受绝经前女性碳水化合物摄入的调节。这一发现可能有助于根据性别和 ABCA1-R230C 基因型设计优化的饮食干预措施。
