Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria 04510, DF Mexico.
Oxid Med Cell Longev. 2011;2011:935160. doi: 10.1155/2011/935160. Epub 2011 Dec 4.
Metals are ubiquitous pollutants present as mixtures. In particular, mixture of arsenic-cadmium-lead is among the leading toxic agents detected in the environment. These metals have carcinogenic and cell-transforming potential. In this study, we used a two step cell transformation model, to determine the role of oxidative stress in transformation induced by a mixture of arsenic-cadmium-lead. Oxidative damage and antioxidant response were determined. Metal mixture treatment induces the increase of damage markers and the antioxidant response. Loss of cell viability and increased transforming potential were observed during the promotion phase. This finding correlated significantly with generation of reactive oxygen species. Cotreatment with N-acetyl-cysteine induces effect on the transforming capacity; while a diminution was found in initiation, in promotion phase a total block of the transforming capacity was observed. Our results suggest that oxidative stress generated by metal mixture plays an important role only in promotion phase promoting transforming capacity.
金属是普遍存在的污染物，通常以混合物的形式存在。特别是，砷-镉-铅混合物是在环境中检测到的主要有毒物质之一。这些金属具有致癌和细胞转化的潜力。在这项研究中，我们使用两步细胞转化模型来确定氧化应激在砷-镉-铅混合物诱导的转化中的作用。测定了氧化损伤和抗氧化反应。金属混合物处理会诱导损伤标志物的增加和抗氧化反应。在促进阶段观察到细胞活力丧失和转化潜能增加。这一发现与活性氧的产生显著相关。用 N-乙酰半胱氨酸共同处理会对转化能力产生影响；而在起始阶段发现减少，在促进阶段观察到转化能力完全被阻断。我们的结果表明，金属混合物产生的氧化应激仅在促进阶段对促进转化能力起重要作用。
