J Biomed Opt. 2011 Dec;16(12):120503. doi: 10.1117/1.3662456.
We have developed a multifocus confocal Raman microspectroscopy system that allows simultaneous analyses of ≈ 80 individual biological or airborne microparticles based on a precise image-guided technique. Multiple individual particles adhered in random positions on a coverslip were illuminated by a multifocus excitation pattern formed by rapidly steering a single laser beam with a pair of galvo-mirrors, and their Raman scatterings were synchronously projected with another galvo-mirror to different rows of a CCD chip for parallel spectroscopic analyses. We show that this technique can be used to rapidly identify single airborne particles or bacteria collected on a slide and to monitor germination dynamics of multiple bacterial spores in real-time.
我们开发了一种多焦点共焦拉曼显微镜系统，该系统允许基于精确的图像引导技术同时分析 ≈ 80 个单个生物或空气传播的微粒。多个单个颗粒随机附着在盖玻片上的位置上，通过一对振镜快速引导单激光束形成多焦点激发图案对其进行照射，并且它们的拉曼散射通过另一个振镜同步投射到 CCD 芯片的不同行以进行平行光谱分析。我们表明，该技术可用于快速识别收集在载玻片上的单个空气传播颗粒或细菌，并实时监测多个细菌孢子的发芽动态。
