Spertino Stefano, Cipriani Valentina, De Angelis Chiara, Giuffrida Maria Gabriella, Marsano Francesco, Cavaletto Maria
Department of Environmental and Life Sciences, University of Piemonte Orientale, Viale T. Michel, 11, 15121 Alessandria, Italy.
Mol Biosyst. 2012 Apr;8(4):967-74. doi: 10.1039/c2mb05400k. Epub 2011 Dec 21.
Upon combining bidimensional electrophoresis with monodimensional separation, a more comprehensive analysis of the milk fat globule membrane has been obtained. The proteomic profile of caprine milk fat globules revealed the presence of butyrophilin, lactadherin and perilipin as the major proteins, they were also associated to bovine and human milk fat globule membranes. Xanthine dehydrogenase/oxidase has been detected only in monodimensional gels. Biological activity of milk fat globules has been evaluated in Caco2-cells, as a representative model of the intestinal barrier. The increase of cell viability was indicative of a potential nutraceutical role for the whole milk fat globule, suggesting a possible employment in milk formula preparation.
将二维电泳与一维分离相结合后，对乳脂肪球膜进行了更全面的分析。山羊乳脂肪球的蛋白质组学图谱显示，主要蛋白质有嗜乳脂蛋白、乳黏附素和围脂滴蛋白，它们也与牛乳和人乳脂肪球膜相关。黄嘌呤脱氢酶/氧化酶仅在一维凝胶中被检测到。作为肠屏障的代表性模型，已在Caco2细胞中评估了乳脂肪球的生物活性。细胞活力的增加表明全乳脂肪球具有潜在的营养保健作用，这暗示了其在奶粉制备中的可能应用。
