Department of Pediatric Clinics, Preventive and Community Dentistry, School of Dentistry, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Am J Orthod Dentofacial Orthop. 2012 Jan;141(1):24-9. doi: 10.1016/j.ajodo.2011.06.036.
Knowing the microbiota that colonizes orthodontic appliances is important for planning strategies and implementing specific preventive measures during treatment. The purpose of this clinical trial was to evaluate in vivo the contamination of metallic orthodontic brackets with 40 DNA probes for different bacterial species by using the checkerboard DNA-DNA hybridization (CDDH) technique.
Eighteen patients, 11 to 29 years of age having fixed orthodontic treatment, were enrolled in the study. Each subject had 2 new metallic brackets bonded to different premolars in a randomized manner. After 30 days, the brackets were removed and processed for analysis by CDDH. Data on bacterial contamination were analyzed descriptively and with the Kruskal-Wallis and Dunn post tests (α = 0.05). Forty microbial species (cariogenic microorganisms, bacteria of the purple, yellow, green, orange complexes, "red complex +Treponema socranskii," and the cluster of Actinomyces) were assessed.
Most bacterial species were present in all subjects, except for Streptococcus constellatus, Campylobacter rectus, Tannerella forsythia, T socranskii, and Lactobacillus acidophillus (94.4%), Propionibacterium acnes I and Eubacterium nodatum (88.9%), and Treponema denticola (77.8%). Among the cariogenic microorganisms, Streptococcus mutans and Streptococcus sobrinus were found in larger numbers than L acidophillus and Lactobacillus casei (P <0.001). The periodontal pathogens of the orange complex were detected in larger numbers than those of the "red complex +T socranskii" (P <0.0001). Among the bacteria not associated with specific pathologies, Veillonella parvula (purple complex) was the most frequently detected strain (P <0.0001). The numbers of yellow and green complex bacteria and the cluster of Actinomyces were similar (P >0.05).
Metallic brackets in use for 1 month were multi-colonized by several bacterial species, including cariogenic microorganisms and periodontal pathogens, reinforcing the need for meticulous oral hygiene and additional preventive measures to maintain oral health in orthodontic patients.
了解定植于正畸矫治器的微生物群落对于治疗期间规划策略和实施特定预防措施非常重要。本临床试验的目的是通过使用斑点杂交 DNA-DNA 杂交（CDDH）技术评估 40 种不同细菌种属的 DNA 探针对金属正畸托槽的体内污染情况。
18 名年龄在 11 至 29 岁之间正在接受固定正畸治疗的患者入组本研究。每个患者以随机方式在不同的前磨牙上粘接 2 个新的金属托槽。30 天后，将托槽取下并用 CDDH 进行处理。使用描述性和 Kruskal-Wallis 和 Dunn 后检验（α=0.05）对细菌污染数据进行分析。评估了 40 种微生物（致龋微生物、紫色、黄色、绿色、橙色复合体细菌、“红色复合体+Treponema socranskii”和放线菌簇）。
除链球菌、直肠弯曲菌、福赛斯坦纳菌、T socranskii 和嗜酸乳杆菌（94.4%）、痤疮丙酸杆菌 I 和内氏放线菌（88.9%）和伴放线放线杆菌（77.8%）外，大多数细菌种属均存在于所有受试者中。在致龋微生物中，变形链球菌和远缘链球菌的数量多于嗜酸乳杆菌和干酪乳杆菌（P<0.001）。橙色复合体的牙周病原体的检出数量多于“红色复合体+T socranskii”（P<0.0001）。在与特定疾病无关的细菌中，普氏栖牙菌（紫色复合体）是最常检测到的菌株（P<0.0001）。黄色和绿色复合体细菌以及放线菌簇的数量相似（P>0.05）。
使用 1 个月的金属托槽被多种细菌定植，包括致龋微生物和牙周病原体，这强调了需要对正畸患者进行精心的口腔卫生和额外的预防措施以维持口腔健康。
