State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
Biomaterials. 2012 Mar;33(8):2583-92. doi: 10.1016/j.biomaterials.2011.12.014. Epub 2011 Dec 21.
In this study, we design a controlled release system based on CaF(2):Ce(3+)/Tb(3+)-poly(acrylic acid) (PAA) composite microspheres, which were fabricated by filling the pH-responsive PAA inside CaF(2):Ce(3+)/Tb(3+) hollow spheres via photopolymerization route. The CaF(2):Ce(3+)/Tb(3+) hollow spheres prepared by hydrothermal route possess mesoporous structure and show strong green fluorescence from Tb(3+) under UV excitation. Doxorubicin hydrochloride (DOX), a widely used anti-cancer drug, was used as a model drug to evaluate the loading and controlled release behaviors of the composite microspheres due to the good biocompatibility of the samples using MTT assay. The composite carriers provide a strongly pH-dependent drug release behavior owing to the intrinsic property of PAA and its interactions with DOX. The endocytosis process of drug-loaded microspheres was observed using confocal laser scanning microscopy (CLSM) and the in vitro cytotoxic effect against SKOV3 ovarian cancer cells of the DOX-loaded carriers was investigated. In addition, the extent of drug release could be monitored by the altering of photoluminescence (PL) intensity of CaF(2):Ce(3+)/Tb(3+). Considering the good biocompatibility, high drug loading content and pH-dependent drug release of the materials, these hybrid luminescent microspheres have potential applications in drug controlled release and disease therapy.
在这项研究中，我们设计了一种基于 CaF(2):Ce(3+)/Tb(3+)-聚(丙烯酸) (PAA) 复合微球的控制释放系统，该系统是通过光聚合途径将 pH 响应性 PAA 填充到 CaF(2):Ce(3+)/Tb(3+) 空心球内制备而成的。水热法制备的 CaF(2):Ce(3+)/Tb(3+) 空心球具有介孔结构，在紫外光激发下，Tb(3+)表现出强烈的绿色荧光。盐酸阿霉素 (DOX) 是一种广泛使用的抗癌药物，被用作模型药物，通过 MTT 试验评估了复合微球的载药和控制释放行为，因为样品具有良好的生物相容性。由于 PAA 的固有性质及其与 DOX 的相互作用，复合载体提供了强烈依赖 pH 的药物释放行为。使用共聚焦激光扫描显微镜 (CLSM) 观察载药微球的内吞过程，并研究了载药载体对 SKOV3 卵巢癌细胞的体外细胞毒性作用。此外，还可以通过改变 CaF(2):Ce(3+)/Tb(3+) 的光致发光 (PL) 强度来监测药物的释放程度。考虑到材料的良好生物相容性、高载药含量和 pH 依赖性药物释放，这些混合发光微球在药物控制释放和疾病治疗方面具有潜在的应用前景。
