纯化的φ6核衣壳能够感染外膜部分受损的宿主细胞并产生子代病毒。

Purified phi 6 nucleocapsids are capable of productive infection of host cells with partially disrupted outer membranes.

作者信息

Ojala P M, Romantschuk M, Bamford D H

机构信息

Department of Genetics, University of Helsinki, Finland.

出版信息

Virology. 1990 Oct;178(2):364-72. doi: 10.1016/0042-6822(90)90333-m.

DOI:10.1016/0042-6822(90)90333-m

PMID:2219699

Abstract

Purified nucleocapsids of bacteriophage phi 6, lacking the phage lipid envelope, are unable to infect intact Pseudomonas syringae host cells. A method for studying the process by which a naked virus particle, the phi 6 nucleocapsid, penetrates the host cytoplasmic membrane was developed. Host cells were rendered competent for nucleocapsid infection by treatment with repeated washings with salt and sucrose and the subsequent addition of lysozyme. This treatment disrupts the outer membrane, permitting the nucleocapsid to reach the cytoplasmic membrane and to infect the cell. The nucleocapsid infection is blocked by monoclonal antibodies raised against the nucleocapsid shell protein P8.

摘要

缺乏噬菌体脂质包膜的噬菌体phi 6的纯化核衣壳无法感染完整的丁香假单胞菌宿主细胞。我们开发了一种方法来研究裸露的病毒颗粒——phi 6核衣壳穿透宿主细胞质膜的过程。通过用盐和蔗糖反复洗涤并随后添加溶菌酶来处理宿主细胞，使其对核衣壳感染具有感受态。这种处理会破坏外膜，使核衣壳能够到达细胞质膜并感染细胞。针对核衣壳壳蛋白P8产生的单克隆抗体可阻断核衣壳感染。