噬菌体phi6中双链RNA基因组片段数量的定向变化。
Directed changes in the number of double-stranded RNA genomic segments in bacteriophage phi6.
作者信息
Onodera S, Qiao X, Qiao J, Mindich L
机构信息
Department of Microbiology, The Public Health Research Institute, New York, NY 10016, USA.
出版信息
Proc Natl Acad Sci U S A. 1998 Mar 31;95(7):3920-4. doi: 10.1073/pnas.95.7.3920.
Bacteriophage Phi6 has a genome of three segments of double-stranded RNA. The segments are designated S, M, and L. Each segment has a unique packaging site, pac, near the 5' end of the plus strand. The plus strands of the segments are normally packaged in the order S, M, L. Chimeras of segment M and S in which segment M is at the 5' end of the plus strand can be stably incorporated into the virion; however, an independent segment S must be included along with normal segment L, even if it contains no active genes. A chimera of segment M and S in which segment S is at the 5' end of the plus strand can be stably incorporated into the virion along with normal segment L to form a two-segment genome. A chimera of segments S, M, and L in which the packaging sequence is that of S can also form a stable nonsegmented genome. These findings are consistent with a model that we have proposed for the packaging of the Phi6 genome.
噬菌体Phi6具有由三段双链RNA组成的基因组。这些片段被命名为S、M和L。每个片段在正链的5'端附近都有一个独特的包装位点pac。片段的正链通常按S、M、L的顺序进行包装。在正链5'端为片段M的片段M和S的嵌合体可以稳定地整合到病毒粒子中；然而，即使独立的片段S不包含活性基因，也必须与正常的片段L一起包含在内。在正链5'端为片段S的片段M和S的嵌合体可以与正常的片段L一起稳定地整合到病毒粒子中，形成两段基因组。包装序列为S的片段S、M和L的嵌合体也可以形成稳定的无片段基因组。这些发现与我们提出的Phi6基因组包装模型一致。
Zotero 插件