Lin Tamar, Duek Or, Dori Amir, Kofman Ora
Department of Psychology and Zlotowski Center for Neuroscience, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Int J Dev Neurosci. 2012 Apr;30(2):113-20. doi: 10.1016/j.ijdevneu.2011.12.004. Epub 2011 Dec 17.
The long-term effect of postnatal administration of a sub-toxic dose of the irreversible acetylcholinesterase inhibitor diisopropylfluorophosphate (DFP) on depression and anxiety behavior was compared in two strains of inbred mice. C57BL/6J and Balb/C mice were injected for 7 consecutive days with either 1 mg/kg DFP or saline on postnatal days 14-20. Mice were tested at age 3-4 months for initial and learned anxiety using double-exposure elevated plus maze and to a novel enclosed environment. Depression was assayed using the sweet preference model of anhedonia and the forced swim test for despair. Postnatal DFP pretreatment led to less activity and more immobility in the elevated plus maze in both mouse strains in the first session. The effect was attenuated in the second session in the C57BL/6J strain but not the Balb/C strain. DFP did not affect the sweet preference or forced swim tests, suggesting a dissociation between the long-term effects of DFP on immobility in the context of approach-avoidance conflict (elevated plus maze) versus despair (forced swim).
在两种近交系小鼠中比较了产后给予亚毒性剂量的不可逆乙酰胆碱酯酶抑制剂二异丙基氟磷酸酯（DFP）对抑郁和焦虑行为的长期影响。C57BL/6J和Balb/C小鼠在出生后第14 - 20天连续7天注射1mg/kg DFP或生理盐水。在3 - 4个月龄时，使用双暴露高架十字迷宫和新的封闭环境对小鼠进行初始和习得性焦虑测试。使用快感缺失的甜味偏好模型和绝望的强迫游泳测试来检测抑郁。产后DFP预处理导致两种小鼠品系在高架十字迷宫的第一阶段活动减少且静止时间增加。在C57BL/6J品系的第二阶段这种效应减弱，但在Balb/C品系中没有减弱。DFP不影响甜味偏好或强迫游泳测试，这表明在接近 - 回避冲突（高架十字迷宫）与绝望（强迫游泳）的背景下，DFP对静止的长期影响之间存在分离。
