一种用于第四条染色体突变克隆分析的新型遗传工具。
A novel genetic tool for clonal analysis of fourth chromosome mutations.
作者信息
Sousa-Neves Rui, Schinaman Joseph M
机构信息
Department of Biology, Case Western Reserve University, Cleveland, OH, USA.
出版信息
Fly (Austin). 2012 Jan-Mar;6(1):49-56. doi: 10.4161/fly.18415. Epub 2012 Jan 1.
Abstract
The fourth chromosome of Drosophila remains one of the most intractable regions of the fly genome to genetic analysis. The main difficulty posed to the genetic analyses of mutations on this chromosome arises from the fact that it does not undergo meiotic recombination, which makes recombination mapping impossible, and also prevents clonal analysis of mutations, a technique which relies on recombination to introduce the prerequisite recessive markers and FLP-recombinase recognition targets (FRT). Here we introduce a method that overcomes these limitations and allows for the generation of single Minute haplo-4 clones of any fourth chromosome mutant gene in tissues of developing and adult flies.
摘要
果蝇的第四条染色体仍然是果蝇基因组中最难进行遗传分析的区域之一。对这条染色体上的突变进行遗传分析的主要困难在于，它不会发生减数分裂重组，这使得重组作图无法进行，同时也阻碍了突变的克隆分析，而克隆分析技术依赖重组来引入必要的隐性标记和FLP重组酶识别靶点（FRT）。在此，我们介绍一种方法，该方法克服了这些限制，能够在发育中的果蝇和成年果蝇的组织中生成任何第四条染色体突变基因的单 Minute 单倍体-4 克隆。
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