Nederlof Michel, Watanabe Shigeo, Burnip Bill, Taylor D Lansing, Critchley-Thorne Rebecca
Cernostics, Inc., 1401 Forbes Avenue, Suite 302, Pittsburgh, PA 15219.
J Pathol Inform. 2011;2:50. doi: 10.4103/2153-3539.89849. Epub 2011 Nov 15.
For many years pathologists have used Hematoxylin and Eosin (H&E), single marker immunohistochemistry (IHC) and in situ hybridization with manual analysis by microscopy or at best simple digital imaging. There is a growing trend to update pathology to a digital workflow to improve objectivity and productivity, as has been done in radiology. There is also a need for tissue-based multivariate biomarker assays to improve the accuracy of diagnostic, prognostic, and predictive testing. Multivariate tests are not compatible with the traditional single marker, manual analysis pathology methods but instead require a digital platform with brightfield and fluorescence imaging, quantitative image analysis, and informatics. Here we describe the use of the Hamamatsu NanoZoomer Digital Pathology slide scanner with HCImage software for combined brightfield and multiplexed fluorescence biomarker analysis and highlight its applications in biomarker research and pathology testing. This combined approach will be an important aid to pathologists in making critical diagnoses.
多年来，病理学家一直使用苏木精和伊红（H&E）染色、单标记免疫组织化学（IHC）以及通过显微镜手动分析或至多简单数字成像的原位杂交技术。与放射学领域一样，将病理学更新为数字工作流程以提高客观性和生产力的趋势日益明显。此外，还需要基于组织的多变量生物标志物检测来提高诊断、预后和预测性检测的准确性。多变量检测与传统的单标记、手动分析病理学方法不兼容，而是需要一个具备明场和荧光成像、定量图像分析及信息学功能的数字平台。在此，我们描述了使用配备HCImage软件的滨松NanoZoomer数字病理切片扫描仪进行明场和多重荧光生物标志物联合分析，并强调其在生物标志物研究和病理检测中的应用。这种联合方法将对病理学家做出关键诊断提供重要帮助。
