University of Freiburg, Department of Microsystems Engineering (IMTEK), Lab of Simulation, Georges-Köhler-Allee 103, 79110 Freiburg, Germany.
Lab Chip. 2012 Feb 7;12(3):495-502. doi: 10.1039/c2lc20585h. Epub 2011 Dec 23.
We present a lab on a chip (LOC) compatible modular platform for magnetic resonance (MR)-based investigation of sub-millimetre samples. The platform combines the advantages offered respectively by microcoils (high resolution at the microscale) and macroscopic surface coils (large field of view) as MR-detectors and consists of a phased array of microcoils (PAMs) providing a flat MR-sensitive area of 18.3 mm(2) with a B(0)-field uniformity better than 0.25 ppm in the sensor centre area. We demonstrate both high-resolution magnetic resonance imaging (MRI) and NMR spectroscopy using this platform. To demonstrate the application for biological samples, we report MR imaging of fish oocytes with an in-plane resolution of 30 × 30 μm(2) and a contrast to noise ratio of 10 for a scan time of only 13 min 39 s. We have also demonstrated high-resolution spectroscopy of a water phantom achieving 11 ppb (4.5 Hz at 400 MHz) linewidth and an SNR of 28 for only 12 s scan time. State of the art automatic wire bonding technology in conjunction with MEMS techniques has been employed to manufacture the platform with potential applications in MR-investigation of planar samples.
我们提出了一种适用于芯片实验室 (LOC) 的模块化平台，用于基于磁共振 (MR) 对亚毫米级样品进行研究。该平台结合了微线圈（在微观尺度上具有高分辨率）和宏观表面线圈（大视野）作为 MR 探测器各自提供的优势，由微线圈相控阵 (PAMs) 组成，提供了一个平坦的 MR 敏感区域，面积为 18.3 平方毫米，在传感器中心区域的 B0 场均匀性优于 0.25 ppm。我们使用该平台展示了高分辨率磁共振成像 (MRI) 和 NMR 光谱学。为了展示对生物样品的应用，我们报告了鱼卵的 MR 成像，其平面分辨率为 30×30μm2，对比度噪声比为 10，扫描时间仅为 13 分 39 秒。我们还展示了水模体的高分辨率光谱学，线宽为 11 ppb（400 MHz 时为 4.5 Hz），信噪比为 28，扫描时间仅为 12 秒。采用最先进的自动引线键合技术与 MEMS 技术相结合，制造了该平台，有望应用于平面样品的 MR 研究。
