金属蛋白酶 2 和 9 及其组织抑制剂 1 和 2 在外阴硬化性苔藓中增加。

Metalloproteinases 2 and 9 and their tissue inhibitors 1 and 2 are increased in vulvar lichen sclerosus.

机构信息

Programa de Biologia Estrutural, Instituto de Bioquímica Médica, Centro Nacional de Ressonância Magnética Nuclear Jiri Jonas, Universidade Federal do Rio de Janeiro, 21941-590 Rio de Janeiro, RJ, Brazil.

出版信息

Eur J Obstet Gynecol Reprod Biol. 2012 Mar;161(1):96-101. doi: 10.1016/j.ejogrb.2011.12.003. Epub 2011 Dec 24.

DOI:10.1016/j.ejogrb.2011.12.003

PMID:22200256

Abstract

OBJECTIVES

To evaluate the expression of different matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in vulvar lichen sclerosus (LS), a chronic dermatosis in women, histologically characterized by a zone of collagen remodeling in the superior dermis.

STUDY DESIGN

Analysis of the expression of different MMPs (MMP-1, -2, -9 and -13) and TIMPs (TIMP-1 and -2) by reverse transcriptase-polymerase chain reaction (RT-PCR) in vulvar biopsies from patients with LS (n=11), classified according to Hewitt histological criteria and compared with clinically normal vulvar tissue (n=5), and the immunohistochemistry of MMP-2 and -9 and TIMP-1 and -2 distribution in the remodeling zone of LS (n=31) and in clinically normal vulvar tissue (n=28).

RESULTS

Although no statistically significant difference between LS and normal skin groups at the mRNA level of MMP and TIMP transcripts was shown, an increase in the immunodistribution of MMP-2 and -9 and TIMP-1 and -2 in LS compared to normal vulvar skin was observed.

CONCLUSIONS

These results suggest that these molecules could be related to the process of cutaneous collagen remodeling in LS pathology.

摘要

目的

评估不同基质金属蛋白酶（MMPs）和金属蛋白酶组织抑制剂（TIMPs）在女性慢性皮肤病外阴硬化性苔藓（LS）中的表达，其组织学特征为真皮上层存在胶原重塑区。

研究设计

通过逆转录-聚合酶链反应（RT-PCR）分析 LS 患者（n=11）外阴活检组织中不同 MMPs（MMP-1、-2、-9 和 -13）和 TIMPs（TIMP-1 和 -2）的表达情况，并根据 Hewitt 组织学标准进行分类，与临床正常外阴组织（n=5）进行比较，并对 LS 重塑区（n=31）和临床正常外阴组织（n=28）中 MMP-2 和 -9 以及 TIMP-1 和 -2 的免疫分布进行免疫组化分析。