Lab. Regulation of Neuron Function, I.P. Pavlov Institute of Physiology of the Russian Academy of Sciences, 199034, nab. Makarova 6, St. Petersburg, Russia.
Eur J Pharmacol. 2012 Feb 29;677(1-3):55-62. doi: 10.1016/j.ejphar.2011.12.004. Epub 2011 Dec 16.
Neurotrophic and neuroprotective properties of Hsp70 were studied in olfactory cortex slices of hypertensive rats under normal conditions and under influence of autologous blood (blood clot). Under normal conditions, synaptic response in slices depended on Hsp70 amount in medium. Incubation of slices with Hsp70 showed a biphasic effect on electrogenesis in tissue. Hsp70 (from 0.1 to 3.0 μg/ml) stimulated glutamatergic and GABA-ergic receptor activity. An inhibitory action on synaptic response is detected at increased amounts of Hsp70 (>4 μg/ml). Pretreatment with Hsp70 (1 and 10 μg/ml) prevented the irreversible inhibition of bioelectric activity in neurons damaged by autologous blood action and interfered with swelling in slices. Our results indicate that exogenous Hsp70 may play a role in the maintenance of neuronal synaptic functions in normal conditions, and protects electrogenesis of brain tissue in strokes.
研究了热休克蛋白 70（Hsp70）在正常条件下和在自体血（血凝块）影响下的高血压大鼠嗅皮层切片中的神经营养和神经保护特性。在正常条件下，切片中的突触反应取决于培养基中 Hsp70 的含量。用 Hsp70 孵育切片对组织中的电发生表现出双相作用。Hsp70（0.1 至 3.0 μg/ml）刺激谷氨酸能和 GABA 能受体活性。在 Hsp70 浓度增加（>4 μg/ml）时，检测到对突触反应的抑制作用。用 Hsp70（1 和 10 μg/ml）预处理可防止自体血作用损伤的神经元的生物电活动不可逆抑制，并干扰切片中的肿胀。我们的结果表明，外源性 Hsp70 可能在正常条件下维持神经元突触功能中发挥作用，并保护中风时脑组织的电发生。
