State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China.
J Invertebr Pathol. 2012 Feb;109(2):260-3. doi: 10.1016/j.jip.2011.12.003. Epub 2011 Dec 17.
The spotted asparagus beetle, Crioceris quatuordecimpunctata (Coleoptera: Chrysomelidae), is one of the most devastating pests of asparagus in China. Sprayed synthetic pesticides have been used to control C. quatuordecimpunctata damage, but they pose problems because of residues and harm to natural enemies. Neither the microbial coleopteran-specific toxin from Bacillus thuringiensistenebrionis, Cry3Aa, nor the fungal pathogen Beauveria bassiana have sufficient activity to effectively control C. quatuordecimpunctata damage to asparagus. However, second instar C. quatuordecimpunctata larvae exposed to a sublethal dose of Cry3Aa toxin demonstrated significantly higher larval mortality when exposed to B. bassiana. Our results suggest that a combination of Cry3Aa and B. bassiana may be effective in reducing damage by C. quatuordecimpunctata larvae to asparagus.
点叶野螟，又称十四点野螟，是中国芦笋生产中最具破坏性的害虫之一。曾使用合成农药喷雾来防治点叶野螟的危害，但由于残留问题和对天敌的伤害，这些农药的使用存在问题。苏云金芽孢杆菌 nembrionis 的昆虫特异性毒素 Cry3Aa 和真菌病原体球孢白僵菌对防治芦笋上的点叶野螟的危害都没有足够的活性。然而，暴露于Cry3Aa 毒素亚致死剂量的第二龄点叶野螟幼虫在接触球孢白僵菌时表现出明显更高的幼虫死亡率。我们的研究结果表明，Cry3Aa 和球孢白僵菌的组合可能有效减少点叶野螟幼虫对芦笋的危害。
