National Center of Competence in Research (NCCR), Neuro Center for Proteomics, UZH/ ETH Zurich, Wolfgang-Pauli-Strasse 16, 8093 Zurich, Switzerland.
Front Biosci (Landmark Ed). 2012 Jan 1;17(5):1599-612. doi: 10.2741/4006.
Communication between cells of the immune system and the organism is dependent on information processing mediated by proteins of the cell surface. The cell surface proteome consists of a group of functionally diverse proteins, which not only enables but also limits the interaction capacities of cells within their particular microenvironment. Although these proteins represent a highly important proteome for immunological research, most routinely used technologies for their detection only allow for a fragmented view of the ensemble of cell surface located proteins. A major bottleneck is the limited availability of high quality antibodies against cell surface protein targets that altogether impedes a Systems Biology view on the cell surface proteome (surfaceome) and its concerted functions during signal processing. Recent developments in mass spectrometry-based technologies enable now complementary approaches for the qualitative and quantitative analysis of the surfaceome. Here, we highlight recent progress in the field towards the identification and quantification of the surfaceome as an important subproteome forming the information gateway of the cell.
细胞间的通讯免疫系统和生物体是依赖于信息处理介导的细胞表面蛋白。细胞表面蛋白质组由一群具有不同功能的蛋白质，它不仅能使，但也限制了细胞的相互作用能力在其特定的微环境中。虽然这些蛋白质是一个非常重要的蛋白质组的免疫学研究，最常用的技术，他们的检测只允许一个破碎的视图ensemble 位于细胞表面的蛋白质。一个主要的瓶颈是有限的高质量的抗体对细胞表面蛋白靶标，共同阻碍了一个系统生物学的观点对细胞表面蛋白质组（表面蛋白质组）及其协同作用的功能在信号处理。最近的发展在基于质谱的技术现在使互补的方法的定性和定量分析的表面蛋白质组。在这里，我们强调了最近的进展在这一领域的鉴定和定量的表面蛋白质组作为一个重要的亚蛋白质组形成的信息网关的单元格。
