Instituto de Biotecnología, CICVyA, CNIA, INTA Castelar, Dr. N. Repetto y Los Reseros s/n, 1686 Hurlingham, Provincia de Buenos Aires, Argentina.
Res Microbiol. 2012 Apr;163(3):221-32. doi: 10.1016/j.resmic.2011.12.001. Epub 2011 Dec 13.
Sequence analysis of the 16S ribosomal RNA gene was used to study bacterial diversity of a pristine forest soil and of two cultures of the same soil enriched with cellulolytic bacteria. Our analysis revealed high bacterial diversity in the native soil sample, evidencing at least 10 phyla, in which Actinobacteria, Proteobacteria and Acidobacteria accounted for more than 76% of all sequences. In both enriched samples, members of Proteobacteria were the most frequently represented. The majority of bacterial genera in both enriched samples were identified as Brevundimonas and Caulobacter, but members of Devosia, Sphingomonas, Variovorax, Acidovorax, Pseudomonas, Xanthomonas, Stenotrophomonas, Achromobacter and Delftia were also found. In addition, it was possible to identify cellulolytic taxa such as Acidothermus, Micromonospora, Streptomyces, Paenibacillus and Pseudomonas, which indicates that this ecosystem could be an attractive source for study of novel enzymes for cellulose degradation.
采用 16S 核糖体 RNA 基因序列分析的方法，研究了原始森林土壤和两种富含纤维素分解菌的相同土壤培养物的细菌多样性。我们的分析表明，原生土壤样本中具有很高的细菌多样性，至少有 10 个门，其中放线菌门、变形菌门和酸杆菌门占所有序列的 76%以上。在两种富集样本中，变形菌门的成员是最常见的。在两种富集样本中，大多数细菌属被鉴定为短杆菌属和柄杆菌属，但也发现了德沃斯氏菌属、鞘氨醇单胞菌属、鞘氨醇单胞菌属、鞘氨醇单胞菌属、鞘氨醇单胞菌属、鞘氨醇单胞菌属、鞘氨醇单胞菌属、鞘氨醇单胞菌属、鞘氨醇单胞菌属、鞘氨醇单胞菌属和鞘氨醇单胞菌属。此外，还可以鉴定出一些纤维素分解菌，如嗜热酸菌属、微单胞菌属、链霉菌属、芽孢杆菌属和假单胞菌属，这表明该生态系统可能是研究纤维素降解新酶的有吸引力的来源。
