Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 80424, Taiwan.
J Proteomics. 2012 Jun 6;75(10):2924-33. doi: 10.1016/j.jprot.2011.12.008. Epub 2011 Dec 20.
Functionalized quantum dots with dopamine dithiocarbamate (QDs-DDTC) were utilized for the first time as an efficient material for the quantification of efavirenz in human plasma of HIV infected patients and rapid identification of microwave tryptic digest proteins (cytochrome c, lysozyme and BSA) by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The synthesized QDs-DDTC was characterized by using spectroscopic (UV-visible, FT-IR and (1)H NMR) and microscopic (SEM and TEM) techniques. Functionalized QDs-DDTC exhibited a high desorption/ionization efficiency for the rapid quantification of small molecules (efavirenz, tobramycin and aspartame) at low-mass region. QDs-DDTC has well ability to trap target species, and capable to transfer laser energy for efficient desorption/ionization of analytes with background-free detection. The use of QDs-DDTC as a matrix provided good linearity for the quantification of small molecules (R(2)=~0.9983), with good reproducibility (RSD<10%), in the analysis of efavirenz in the plasma of HIV infected patients by the standard addition method. We also demonstrated that the use of functionalized QDs-DDTC as affinity probes for the rapid identification of microwave tryptic digested proteins (cytochrome c, lysozyme and BSA) by MALDI-TOF-MS. QDs-DDTC-based MALDI-TOF-MS approach provides simplicity, rapidity, accuracy, and precision for the determination of efavirenz in human plasma of HIV infected patients and rapid identification of microwave tryptic digested proteins. This new material presents a marked advance in the development of matrix-free mass spectrometric methods for the rapid and precise quantitative determination of a variety of molecules. This article is part of a Special Issue entitled: Proteomics: The clinical link.
首次将具有多巴胺二硫代氨基甲酸盐（QDs-DDTC）功能化的量子点作为一种有效的材料，用于对感染 HIV 的患者血浆中的依非韦伦进行定量，并通过基质辅助激光解吸/电离飞行时间质谱法（ MALDI-TOF-MS）快速鉴定微波酶解蛋白（细胞色素 c、溶菌酶和 BSA）。采用光谱（UV-可见、FT-IR 和（1）H NMR）和微观（SEM 和 TEM）技术对合成的 QDs-DDTC 进行了表征。功能化的 QDs-DDTC 对小分子（依非韦伦、妥布霉素和阿斯巴甜）的快速定量表现出高解吸/电离效率，可在低质量区域进行。QDs-DDTC 具有很好的捕获目标物质的能力，并且能够转移激光能量，从而有效地解吸/电离背景自由检测的分析物。使用 QDs-DDTC 作为基质，通过标准添加法在分析 HIV 感染患者血浆中的依非韦伦时，为小分子的定量提供了良好的线性（R（2）=~0.9983），具有良好的重现性（RSD<10%）。我们还证明，使用功能化的 QDs-DDTC 作为亲和探针，通过 MALDI-TOF-MS 快速鉴定微波酶解蛋白（细胞色素 c、溶菌酶和 BSA）。基于 QDs-DDTC 的 MALDI-TOF-MS 方法为测定 HIV 感染患者血浆中的依非韦伦和快速鉴定微波酶解蛋白提供了简单、快速、准确和精确的方法。这种新材料在发展无基质质谱方法方面取得了显著进展，可用于快速准确地定量测定各种分子。本文是专题的一部分：蛋白质组学：临床联系。
