OIE Reference Laboratory for WTD, PG and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam 632 509, Tamil Nadu, India.
Chemosphere. 2012 Mar;87(1):55-61. doi: 10.1016/j.chemosphere.2011.11.056. Epub 2011 Dec 26.
Cell lines of Etroplus suratensis established in our laboratory were evaluated for their potential use as screening tools for the ecotoxicological assessment of tannery effluent. The cytotoxic effect of tannery effluent in three cell lines derived from eye, kidney and gill tissue of E. suratensis was assessed using multiple endpoints such as Neutral Red (NR) assay, Coomassie Blue (CB) protein assay and Alamar Blue (AB) assay. Acute toxicity tests on fish were conducted by exposing E. suratensis for 96 h to tannery effluent under static conditions. The toxic effect of tannery effluent on the survival of fish was found to be concentration and time dependent. The tannery effluent at the concentration of 15% caused 100% mortality at 96 h whereas the lower concentration (0.5%) caused 13.33% mortality. The cytotoxicity of tannery effluent was found to be similar in the three cell lines tested, independent of the toxic endpoints employed. EC(50) values, the effective concentration of tannery effluent resulting in 50% inhibition of cytotoxicity parameters after 48 h exposure to tannery effluent were calculated for eye, kidney and gill cell lines using NR uptake, AB and cell protein assays. Statistical analysis revealed good correlation with r(2)=0.95-0.99 for all combinations between endpoints employed. Linear correlations between each in vitro EC(50) and the in vivo LC(50) data, were highly significant p<0.001 with r(2)=0.977, 0.968 and 0.906 for AB(50), NR(50), and CB(50), respectively.
在本实验室建立的斜带石斑鱼细胞系被评估为用于制革废水生态毒理学评估的筛选工具的潜在用途。使用多种终点，如中性红（NR）测定、考马斯亮蓝（CB）蛋白测定和阿尔玛蓝（AB）测定，评估制革废水中三种来源于斜带石斑鱼眼、肾和鳃组织的细胞系的细胞毒性效应。在静态条件下，将斜带石斑鱼暴露于制革废水中 96 小时，进行鱼类急性毒性测试。制革废水中的毒性效应与鱼的存活率呈浓度和时间依赖性。在 96 小时时，浓度为 15%的制革废水导致 100%的死亡率，而较低浓度（0.5%）导致 13.33%的死亡率。制革废水的细胞毒性在测试的三种细胞系中是相似的，与所使用的毒性终点无关。使用 NR 摄取、AB 和细胞蛋白测定，在暴露于制革废水 48 小时后，计算制革废水导致 50%抑制细胞毒性参数的有效浓度（EC 50 ）值，用于眼、肾和鳃细胞系。统计分析显示，在所有终点之间的组合中，r 2 =0.95-0.99，相关性良好。在体外 EC 50 和体内 LC 50 数据之间存在线性相关性，具有高度显著性 p<0.001，r 2 =0.977、0.968 和 0.906 分别为 AB(50)、NR(50)和 CB(50)。
