Department of Biomedical Engineering, National Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka 565-8565, Japan.
Biomaterials. 2012 Mar;33(8):2439-48. doi: 10.1016/j.biomaterials.2011.11.075. Epub 2011 Dec 27.
A water-soluble magnetic resonance imaging (MRI) contrast agent, Dextran mono-N-succinimidyl 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate-gadolinium(3+) (Dex-DOTA-Gd(3+)), was shown to enable monitoring of the anatomical migration and the survival period of transplanted stem cells for up to 1 month. Gadolinium molecules in the cells were rapidly eliminated from the site and excreted upon cell death. Endothelial progenitor cells (EPCs) transplanted into the inguinal femoral muscle of rats migrated distally through the knee in rats after hindlimb ischemia but did not migrate in non-ischemic rats. Interestingly, the survival period of transplanted EPCs was notably prolonged in the ischemic limb, indicating that EPCs are required by the ischemic tissues and that the fate of transplanted EPCs was affected by the disease. Compared to the commonly used particle type of MRI contrast agents, the system described in this study is expected to be invaluable to help clarifying the process of stem cell transplantation therapy.
一种水溶性磁共振成像（MRI）造影剂，葡聚糖单琥珀酰亚胺基 1,4,7,10-四氮杂环十二烷-1,4,7,10-四乙酸钆（3+）（Dex-DOTA-Gd（3+）），被证明能够监测移植干细胞的解剖迁移和存活期长达 1 个月。细胞内的镓分子在细胞死亡时迅速从原位排出并排出体外。内皮祖细胞（EPC）移植到大鼠的腹股沟股肌后，在大鼠后肢缺血后通过膝关节向远端迁移，但在非缺血大鼠中不迁移。有趣的是，移植 EPC 的存活期在缺血肢体中显著延长，表明 EPC 是缺血组织所必需的，并且移植 EPC 的命运受到疾病的影响。与常用的 MRI 造影剂颗粒类型相比，本研究中描述的系统有望对阐明干细胞移植治疗过程具有重要意义。
