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利用 Illumina 深度测序研究干旱胁迫和复水生长条件下白芥叶片的基因表达谱。

Gene expression profiling of Sinapis alba leaves under drought stress and rewatering growth conditions with Illumina deep sequencing.

机构信息

Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Oil Crop Biology and Genetic Breeding of the Ministry of Agriculture, Wuhan, 430062, China.

出版信息

Mol Biol Rep. 2012 May;39(5):5851-7. doi: 10.1007/s11033-011-1395-9. Epub 2011 Dec 30.

Abstract

Sinapis alba has many desirable agronomic traits including tolerance to drought. In this investigation, we performed the genome-wide transcriptional profiling of S. alba leaves under drought stress and rewatering growth conditions in an attempt to identify candidate genes involved in drought tolerance, using the Illumina deep sequencing technology. The comparative analysis revealed numerous changes in gene expression level attributable to the drought stress, which resulted in the down-regulation of 309 genes and the up-regulation of 248 genes. Gene ontology analysis revealed that the differentially expressed genes were mainly involved in cell division and catalytic and metabolic processes. Our results provide useful information for further analyses of the drought stress tolerance in Sinapis, and will facilitate molecular breeding for Brassica crop plants.

摘要

白芥具有许多理想的农艺性状,包括耐旱性。在这项研究中,我们使用 Illumina 深度测序技术,对干旱胁迫和复水生长条件下白芥叶片进行了全基因组转录组分析,试图鉴定参与耐旱性的候选基因。比较分析显示,由于干旱胁迫,基因表达水平发生了许多变化,导致 309 个基因下调和 248 个基因上调。基因本体论分析表明,差异表达基因主要参与细胞分裂和催化及代谢过程。我们的研究结果为进一步分析芥菜的耐旱性提供了有用的信息,并将促进十字花科作物的分子育种。

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