Department of Breast Surgery, General Surgery, The First Hospital of China Medical University, Liaoning Province, Shenyang, 110001, China.
Mol Biol Rep. 2012 May;39(5):5875-81. doi: 10.1007/s11033-011-1398-6. Epub 2011 Dec 30.
The aim is to investigate the clinical implications of the Oct-4 and Nestin protein in human breast cancers. A total of 346 cases including 26 fresh and 320 paraffin-embedded tumor tissues were selected for characterizing the frequency of CD44(+)CD24(-) tumor cells by flow cytometry and the differential expression of the stem cell-related genes between CD44(+)CD24(-) and non-CD44(+)CD24(-) tumor cells was analyzed by PCR Array and immunofluorescence. In comparison with the non-CD44(+)CD24(-) tumor cells, the CD44(+)CD24(-), particularly for those with high percentage of Oct-4(+) and Nestin(+), tumor cells had higher tumorigenicity by forming mammospheres in vitro. More importantly, 42 (13.125%) out of 320 tumor tissues were positive for Oct-4 and Nestin staining. Universal analysis and multivariate analysis revealed that the expression of Oct-4 and Nestin was associated significantly with younger age, pathogenic degrees, lymph node metastasis and triple-negative breast cancer independently (P < 0.05) as well as shorter survival (P = 0.001). Oct-4 and Nestin were important regulators of the development of breast cancer, and Oct-4 and Nestin may be used as predictors for the prognosis of breast cancers.
目的是研究 Oct-4 和 Nestin 蛋白在人乳腺癌中的临床意义。共选择了 346 例病例，包括 26 例新鲜和 320 例石蜡包埋肿瘤组织，通过流式细胞术对 CD44(+)CD24(-)肿瘤细胞的频率进行特征描述，并通过 PCR 阵列和免疫荧光分析 CD44(+)CD24(-)和非 CD44(+)CD24(-)肿瘤细胞之间干细胞相关基因的差异表达。与非 CD44(+)CD24(-)肿瘤细胞相比，CD44(+)CD24(-)，特别是那些 Oct-4(+)和 Nestin(+)百分比高的肿瘤细胞，通过体外形成乳腺球体具有更高的致瘤性。更重要的是，320 例肿瘤组织中有 42 例（13.125%）对 Oct-4 和 Nestin 染色呈阳性。通用分析和多变量分析显示，Oct-4 和 Nestin 的表达与年龄较小、病理程度、淋巴结转移和三阴性乳腺癌独立相关（P<0.05），以及生存时间较短（P=0.001）。Oct-4 和 Nestin 是乳腺癌发生发展的重要调节因子，Oct-4 和 Nestin 可能作为乳腺癌预后的预测因子。
