Department of Biology, York University, Faculty of Science and Engineering, Bethune College, Toronto, Ontario, Canada.
Cell Mol Biol Lett. 2012 Mar;17(1):153-70. doi: 10.2478/s11658-011-0039-3. Epub 2011 Dec 28.
P(5) ATPases (ATP13A1 through ATP13A5) are found in all eukaryotes. They are currently poorly characterized and have unknown substrate specificity. Recent evidence has linked two P(5) ATPases to diseases of the nervous system, suggesting possible importance of these proteins within the nervous system. In this study we determined the relative expression of mouse P5 ATPases in development using quantitative real time PCR. We have shown that ATP13A1 and ATP13A2 were both expressed similarly during development, with the highest expression levels at the peak of neurogenesis. ATP13A3 was expressed highly during organogenesis with one of its isoforms playing a more predominant role during the period of neuronal development. ATP13A5 was expressed most highly in the adult mouse brain. We also assessed the expression of these genes in various regions of the adult mouse brain. ATP13A1 to ATP13A4 were expressed differentially in the cerebral cortex, hippocampus, brainstem and cerebellum while levels of ATP13A5 were fairly constant between these brain regions. Moreover, we demonstrated expression of the ATP13A4 protein in the corresponding brain regions using immunohistochemistry. In summary, this study furthers our knowledge of P(5)-type ATPases and their potentially important role in the nervous system.
P(5)ATP 酶（ATP13A1 到 ATP13A5）存在于所有真核生物中。它们目前的特征描述不完整，底物特异性未知。最近的证据将两种 P(5)ATP 酶与神经系统疾病联系起来，这表明这些蛋白质在神经系统中可能具有重要作用。在这项研究中，我们使用定量实时 PCR 测定了发育过程中小鼠 P5ATP 酶的相对表达。我们已经表明，ATP13A1 和 ATP13A2 在发育过程中的表达相似，在神经发生高峰期表达水平最高。ATP13A3 在器官发生过程中高度表达，其同工型之一在神经元发育期间发挥更主要的作用。ATP13A5 在成年小鼠脑中表达最高。我们还评估了这些基因在成年小鼠大脑的不同区域中的表达。ATP13A1 到 ATP13A4 在大脑皮层、海马体、脑干和小脑中有差异表达，而 ATP13A5 的水平在这些脑区之间相当稳定。此外，我们使用免疫组织化学法证明了 ATP13A4 蛋白在相应脑区的表达。总之，这项研究进一步了解了 P(5)型 ATP 酶及其在神经系统中的潜在重要作用。
