Yang Lin, Fu Feng-Ling, Fu Feng-Ling, Li Wan-Chen
Maize Research Institute, Sichuan Agricultural University, Chengdu, China.
Yi Chuan. 2011 Dec;33(12):1327-34. doi: 10.3724/sp.j.1005.2011.01327.
The genetic transformation mediated by Agrobacterium tumefaciens has been widely applied to research of transgenic plants. As the vector of the exotic genes, the integration patterns of T-DNA fragments affects not only transformation efficiency and stability, but also expression properties of the transgenes. This review summaries the two major patterns and the rules of T-DNA integration in Agrobacterim-mediated transformation, rules of T-DNA mediated by Agrobacterium tumefaciens, as well as research tools for flanking sequence amplification. It is attempted to provide references for researches on transformation and T-DNA integration mutation mediated by Agrobacterium tumefaciens.
根癌农杆菌介导的遗传转化已广泛应用于转基因植物研究。作为外源基因的载体，T-DNA片段的整合模式不仅影响转化效率和稳定性，还影响转基因的表达特性。本文综述了农杆菌介导转化中T-DNA整合的两种主要模式及规律、根癌农杆菌介导的T-DNA规律，以及侧翼序列扩增的研究工具。旨在为根癌农杆菌介导的转化及T-DNA整合突变研究提供参考。
