Key Laboratory of Urban Stormwater System and Water Environment, R&D Centre for Sustainable Environmental Biotechnology, Beijing University of Civil Engineering and Architecture, Ministry of Education, Beijing 100044, PR China.
Water Res. 2012 Mar 15;46(4):1251-9. doi: 10.1016/j.watres.2011.12.029. Epub 2011 Dec 20.
As bacterial decay consists of cell death and activity decay, and the corresponding information about AOB/NOB, OHO, PAOs and GAOs has been experimentally acquired, another functional type of bacteria in biological wastewater treatment, methanogens, remains to be investigated, to gather the same information, which is extremely important for such bacteria with low growth rates. With successfully selection and enrichment of both aceticlastic and hydrogenotrophic methanogens, and by means of measuring specific methane activity (SMA) and hydrogen consumption rate (HCR), a series of decay experiments and molecular techniques such as FISH verification and LIVE/DEAD staining revealed, identified and calculated the decay and death rates of both aceticlastic and hydrogenotrophic methanogens respectively. The results indicated that the decay rates of aceticlastic and hydrogenotrophic methanogens were 0.070 and 0.034 d(-1) respectively, and the death rates were thus calculated at 0.022 and 0.016 d(-1) respectively. For this reason, cell deaths were only responsible for 31% and 47% of the total bacterial decay of aceticlastic and hydrogenotrophic methanogens, and activity decays actually contributed significantly to the total bacterial decay, respectively at 69% and 53%.
由于细菌的衰减包括细胞死亡和活性衰减，并且已经通过实验获得了有关 AOB/NOB、OHO、PAOs 和 GAOs 的相应信息，因此，生物废水处理中的另一种功能类型的细菌——产甲烷菌，仍有待研究，以收集相同的信息，这对于生长速度较慢的细菌来说非常重要。通过成功地选择和富集乙酸营养型和氢营养型产甲烷菌，并通过测量特定甲烷活性 (SMA) 和氢消耗率 (HCR)，进行了一系列衰减实验和分子技术，如 FISH 验证和 LIVE/DEAD 染色，分别揭示、鉴定和计算了乙酸营养型和氢营养型产甲烷菌的衰减和死亡率。结果表明，乙酸营养型和氢营养型产甲烷菌的衰减率分别为 0.070 和 0.034 d(-1)，因此死亡率分别计算为 0.022 和 0.016 d(-1)。因此，细胞死亡仅分别占乙酸营养型和氢营养型产甲烷菌总细菌衰减的 31%和 47%，而活性衰减分别对总细菌衰减的贡献分别为 69%和 53%。
