基于 BSA 稳定的金纳米簇的高灵敏度荧光检测胰蛋白酶。
Highly sensitive fluorescent detection of trypsin based on BSA-stabilized gold nanoclusters.
机构信息
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.
出版信息
Biosens Bioelectron. 2012 Feb 15;32(1):297-9. doi: 10.1016/j.bios.2011.12.007. Epub 2011 Dec 13.
In this study, fluorescent metal nanoclusters are presented as novel probes for sensitive detection of protease for the first time. The sensing mechanism is based on trypsin digestion of the protein template of BSA-stabilized Au nanoclusters. The decrease in fluorescence intensity of BSA-Au nanoclusters caused by trypsin allows the sensitive detection of trypsin in the range of 0.01-100 μg/mL. The detection limit for trypsin is 2 ng/mL (86 pM) at a signal-to-noise ratio of 3. The present nanosensor for trypsin detection possesses red emission, excellent biocompatibility, high selectivity, and good stability. In addition, we demonstrated the application of the present approach in real urine samples, which suggested its potential for diagnostic purposes.
在这项研究中，荧光金属纳米团簇首次被用作蛋白酶灵敏检测的新型探针。该传感机制基于牛血清白蛋白稳定的 Au 纳米团簇的蛋白质模板被胰蛋白酶消化。由于胰蛋白酶的存在，BSA-Au 纳米团簇的荧光强度降低，从而可以在 0.01-100 μg/mL 的范围内对胰蛋白酶进行灵敏检测。胰蛋白酶的检测限为 2 ng/mL（86 pM），信噪比为 3。该用于检测胰蛋白酶的纳米传感器具有红色发射、良好的生物相容性、高选择性和良好的稳定性。此外，我们还证明了该方法在实际尿液样本中的应用，表明其在诊断方面具有潜在应用价值。
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