School of Pharmacy, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK.
Biochem Biophys Res Commun. 2012 Feb 3;418(1):17-21. doi: 10.1016/j.bbrc.2011.12.081. Epub 2011 Dec 22.
The molecular signalling pathway of cell migration and whether it can occur independently of the release of intracellular calcium is still not completely understood. Therefore we investigated here the molecular mechanisms of CCL3 induced cell migration and the importance of intracellular calcium for chemotaxis in more detail. We show that CCL3 induced cell migration is dependent on activation of PLC. Several PKC inhibitors block the release of intracellular calcium independently of CCL3 activation and do not affect cell migration. This confirms that the release of intracellular calcium is not necessary for chemotaxis towards CCL3 and that PKC inhibitors should be used with caution in calcium release assays.
细胞迁移的分子信号通路，以及其是否可以独立于细胞内钙离子的释放而发生，目前仍不完全清楚。因此，我们在这里更详细地研究了 CCL3 诱导的细胞迁移的分子机制以及细胞内钙离子对趋化作用的重要性。我们表明，CCL3 诱导的细胞迁移依赖于 PLC 的激活。几种 PKC 抑制剂可独立于 CCL3 的激活阻断细胞内钙离子的释放，并且不影响细胞迁移。这证实了细胞内钙离子的释放对于 CCL3 的趋化作用并非必需，并且在钙释放测定中应谨慎使用 PKC 抑制剂。
