Van Wormhoudt Alain, Gaume Béatrice, Le Bras Yvan, Roussel Valérie, Huchette Sylvain
CNRS UMR 7208, Station de Biologie Marine du Muséum National d'Histoire Naturelle, 29900 Concarneau, France.
Genetica. 2011 Oct;139(10):1217-27. doi: 10.1007/s10709-011-9623-8. Epub 2011 Dec 31.
Analysis of the 18S rDNA sequences of Haliotis tuberculata tuberculata and H. t. coccinea subtaxa identified two different types of 18S rDNA genes and ITS1 regions. These two different genes were also detected in H. marmorata, H. rugosa and H. diversicolor that are separated from H. tuberculata by 5-65 mya. The mean divergence value between type I and type II sequences ranged from 7.25% for 18S to 80% for ITS1. ITS1 type II is homologous with the ITS1 consensus sequences published for many abalone species, whereas ITS1 type I presented only minor homology with a unique database entry for H. iris ITS1. A phylogenetic analysis makes a clear separation between type I and type II ITS1 sequences and supports grouping H. t. tuberculata, H. t. coccinea and H. marmorata together. The two subtaxa do not show any significant differences between the homologous 18S rDNA sequences. A general structure of the ITS1 transcript was proposed, with four major helices for the two types. The two genes were expressed and, for the first time, a putative differential expression of ITS1 type I was detected in the gills, digestive gland and gonads whereas ITS1 type II was expressed in all tissues.
对瘤珠母贝（Haliotis tuberculata tuberculata）和红珠母贝（H. t. coccinea）亚种的18S rDNA序列分析鉴定出两种不同类型的18S rDNA基因和ITS1区域。在与瘤珠母贝在500万至6500万年前分化的黑唇鲍（H. marmorata）、皱纹盘鲍（H. rugosa）和杂色鲍（H. diversicolor）中也检测到了这两种不同的基因。I型和II型序列之间的平均分歧值范围从18S的7.25%到ITS1的80%。II型ITS1与许多鲍鱼物种公布的ITS1共有序列同源，而I型ITS1与虹鲍（H. iris）ITS1的一个独特数据库条目仅呈现出较小的同源性。系统发育分析清楚地将I型和II型ITS1序列分开，并支持将瘤珠母贝、红珠母贝和黑唇鲍归为一组。这两个亚种在同源18S rDNA序列之间没有显示出任何显著差异。提出了ITS1转录本的一般结构，两种类型有四个主要螺旋。这两种基因都有表达，并且首次在鳃、消化腺和性腺中检测到I型ITS1的假定差异表达，而II型ITS1在所有组织中都有表达。
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