Merchant Sabeeha S, Prochnik Simon E, Vallon Olivier, Harris Elizabeth H, Karpowicz Steven J, Witman George B, Terry Astrid, Salamov Asaf, Fritz-Laylin Lillian K, Maréchal-Drouard Laurence, Marshall Wallace F, Qu Liang-Hu, Nelson David R, Sanderfoot Anton A, Spalding Martin H, Kapitonov Vladimir V, Ren Qinghu, Ferris Patrick, Lindquist Erika, Shapiro Harris, Lucas Susan M, Grimwood Jane, Schmutz Jeremy, Cardol Pierre, Cerutti Heriberto, Chanfreau Guillaume, Chen Chun-Long, Cognat Valérie, Croft Martin T, Dent Rachel, Dutcher Susan, Fernández Emilio, Fukuzawa Hideya, González-Ballester David, González-Halphen Diego, Hallmann Armin, Hanikenne Marc, Hippler Michael, Inwood William, Jabbari Kamel, Kalanon Ming, Kuras Richard, Lefebvre Paul A, Lemaire Stéphane D, Lobanov Alexey V, Lohr Martin, Manuell Andrea, Meier Iris, Mets Laurens, Mittag Maria, Mittelmeier Telsa, Moroney James V, Moseley Jeffrey, Napoli Carolyn, Nedelcu Aurora M, Niyogi Krishna, Novoselov Sergey V, Paulsen Ian T, Pazour Greg, Purton Saul, Ral Jean-Philippe, Riaño-Pachón Diego Mauricio, Riekhof Wayne, Rymarquis Linda, Schroda Michael, Stern David, Umen James, Willows Robert, Wilson Nedra, Zimmer Sara Lana, Allmer Jens, Balk Janneke, Bisova Katerina, Chen Chong-Jian, Elias Marek, Gendler Karla, Hauser Charles, Lamb Mary Rose, Ledford Heidi, Long Joanne C, Minagawa Jun, Page M Dudley, Pan Junmin, Pootakham Wirulda, Roje Sanja, Rose Annkatrin, Stahlberg Eric, Terauchi Aimee M, Yang Pinfen, Ball Steven, Bowler Chris, Dieckmann Carol L, Gladyshev Vadim N, Green Pamela, Jorgensen Richard, Mayfield Stephen, Mueller-Roeber Bernd, Rajamani Sathish, Sayre Richard T, Brokstein Peter, Dubchak Inna, Goodstein David, Hornick Leila, Huang Y Wayne, Jhaveri Jinal, Luo Yigong, Martínez Diego, Ngau Wing Chi Abby, Otillar Bobby, Poliakov Alexander, Porter Aaron, Szajkowski Lukasz, Werner Gregory, Zhou Kemin, Grigoriev Igor V, Rokhsar Daniel S, Grossman Arthur R
Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, CA 90095, USA.
Science. 2007 Oct 12;318(5848):245-50. doi: 10.1126/science.1143609.
Chlamydomonas reinhardtii is a unicellular green alga whose lineage diverged from land plants over 1 billion years ago. It is a model system for studying chloroplast-based photosynthesis, as well as the structure, assembly, and function of eukaryotic flagella (cilia), which were inherited from the common ancestor of plants and animals, but lost in land plants. We sequenced the approximately 120-megabase nuclear genome of Chlamydomonas and performed comparative phylogenomic analyses, identifying genes encoding uncharacterized proteins that are likely associated with the function and biogenesis of chloroplasts or eukaryotic flagella. Analyses of the Chlamydomonas genome advance our understanding of the ancestral eukaryotic cell, reveal previously unknown genes associated with photosynthetic and flagellar functions, and establish links between ciliopathy and the composition and function of flagella.
莱茵衣藻是一种单细胞绿藻，其谱系在10亿多年前就与陆地植物分道扬镳。它是研究基于叶绿体的光合作用以及真核生物鞭毛（纤毛）的结构、组装和功能的模型系统，这些鞭毛是从动植物的共同祖先遗传而来，但在陆地植物中丢失了。我们对莱茵衣藻约1.2亿碱基对的核基因组进行了测序，并进行了比较系统基因组分析，鉴定出编码可能与叶绿体或真核生物鞭毛的功能和生物发生相关的未表征蛋白质的基因。对莱茵衣藻基因组的分析增进了我们对原始真核细胞的理解，揭示了与光合作用和鞭毛功能相关的先前未知的基因，并建立了纤毛病与鞭毛的组成和功能之间的联系。
