Duque-Bonisoli C, Salvador A, Díaz T, Contreras-Solis I
Facultad de Ciencias Veterinarias, Universidad Central de Venezuela, Maracay, Aragua, Venezuela.
Reprod Domest Anim. 2012 Dec;47(6):e79-82. doi: 10.1111/j.1439-0531.2011.01974.x. Epub 2011 Dec 29.
The objective of this study was to assess the efficacy of two reduced doses vs a high/luteolytic dose of cloprostenol on luteolytic activity and synchronization of oestrus in cyclic goats. Experiment 1, included 24 goats randomly allocated to three groups: control group (group H) received a single high dose of cloprostenol (87.5 μg; 1.0 ml; i.m.) and M and L groups, which received half (43.75 μg; 0.5 ml) and a third (26.25 μg; 0.3 ml) of the highest dose, respectively. Experiment 2, included 24 goats randomly assigned to the same experimental groups. Each group was treated using two injections of cloprostenol administered 10 days apart to synchronize oestrus. Transrectal ultrasonographic scanning (US) was performed to detect the presence, size and development of corpora lutea and ovarian follicles. Furthermore, detection of oestrus was performed every 12 h between 24 and 72 h after the second injection of cloprostenol, and the luteolytic effect was verified by US. In Experiment 1, all goats that had corpora lutea at timing of treatment regressed their corpora lutea. In Experiment 2, the occurrence of oestrus and the interval between treatment to onset of oestrus were: 100%, 49.5 ± 3.0 h; 100%, 51.0 ± 3.0 h; and 75%, 56.0 ± 3.5 h for H, M and L groups, respectively. The development of preovulatory follicles and occurrence of subsequent corpora lutea were similar among groups. In summary, the use of 26.25 μg of cloprostenol is effective for the synchronization of oestrus in cyclic goats.
本研究的目的是评估两种降低剂量与高/溶黄体剂量的氯前列醇对周期性发情山羊黄体溶解活性和发情同步化的效果。实验1包括24只山羊，随机分为三组：对照组（H组）接受单次高剂量氯前列醇（87.5μg；1.0ml；肌肉注射），M组和L组分别接受最高剂量的一半（43.75μg；0.5ml）和三分之一（26.25μg；0.3ml）。实验2包括24只山羊，随机分配到相同的实验组。每组使用两次间隔10天注射氯前列醇来同步发情。进行经直肠超声扫描（US）以检测黄体和卵巢卵泡的存在、大小和发育情况。此外，在第二次注射氯前列醇后24至72小时内每12小时进行一次发情检测，并通过超声检查验证黄体溶解效果。在实验1中，所有在治疗时具有黄体的山羊其黄体均退化。在实验2中，H组、M组和L组发情发生率及治疗至发情开始的间隔时间分别为：100%，49.5±3.0小时；100%，51.0±3.0小时；75%，56.0±3.5小时。各组排卵前卵泡的发育及随后黄体的出现情况相似。总之，使用26.25μg氯前列醇对周期性发情山羊的发情同步化有效。
