Djurdjić V, Mandić L
Faculty of Chemistry, University of Belgrade, IOHBIA, Yugoslavia.
Anal Biochem. 1990 Jul;188(1):222-7. doi: 10.1016/0003-2697(90)90556-o.
A very suitable spectrophotometric method for simultaneous determination of protein-bound hexoses and fucose is presented. A mixture of L-cysteine and phenol in sulfuric acid was used as reagent, whereas absorption measurements were carried out at two wavelengths, namely at 398 nm for fucose and 490 nm for hexoses determination. Optimum conditions for the application of the method were established, special attention being paid to the possible interference of fucose determination with that of hexoses, and vice versa. The proposed method was applied to the determination of hexoses and fucose in serum glycoproteins and seromucoids. The method was found to be very practical enabling a simultaneous determination of both kinds of carbohydrates; moreover, it was proved to be more sensitive and specific in comparison with methods commonly used for individual determination of fucose with L-cysteine and hexoses with phenol.
本文提出了一种非常适合同时测定蛋白质结合己糖和岩藻糖的分光光度法。以L-半胱氨酸和苯酚在硫酸中的混合物作为试剂，而吸收测量在两个波长下进行，即398nm用于测定岩藻糖，490nm用于测定己糖。确定了该方法应用的最佳条件，特别关注了岩藻糖测定对己糖测定的可能干扰，反之亦然。所提出的方法应用于血清糖蛋白和血清类粘蛋白中己糖和岩藻糖的测定。结果发现该方法非常实用，能够同时测定两种碳水化合物；此外，与常用的分别用L-半胱氨酸测定岩藻糖和用苯酚测定己糖的方法相比，该方法被证明更灵敏、更具特异性。
