Stein M A, McAllister S A, Johnston K H, Diedrich D L
Department of Microbiology, Immunology and Parasitology, Louisiana State University Medical Center, New Orleans 70112-1393.
Anal Biochem. 1990 Aug 1;188(2):285-7. doi: 10.1016/0003-2697(90)90607-b.
Bacterial lipopolysaccharide (LPS) blotted to polyvinylidene difluoride (PVDF) membranes was detected by a technique adapted from current methodologies used to detect glycoproteins. PVDF-bound LPS was coupled to a hapten and localized on the membrane by Western blotting with an antibody-alkaline phosphatase conjugate specific for the hapten. Immobilon blots could be made reversibly transparent for photography and densitometry.
通过一种改编自当前用于检测糖蛋白的方法来检测印迹在聚偏二氟乙烯（PVDF）膜上的细菌脂多糖（LPS）。将与PVDF结合的LPS与一种半抗原偶联，并通过用对该半抗原特异的抗体 - 碱性磷酸酶偶联物进行蛋白质印迹法在膜上定位。Immobilon印迹可以可逆地变透明以用于摄影和光密度测定。
