Centre for Biomaterial Development and Berlin-Brandenburg, Centre for Regenerative Therapies, Institute of Polymer Research, Helmholtz-Zentrum Geesthacht, Teltow, Germany.
Clin Hemorheol Microcirc. 2011;49(1-4):423-30. doi: 10.3233/CH-2011-1492.
So called intermediate (MO2) monocytes/macrophages possess anti-inflammatory properties and express the MO lineage marker CD163. On a hydrophilic, acrylamide-based hydrogel human intermediate (CD14++ CD16+) CD163++ monocytes/macrophages (aMO2) which were angiogenically stimulated, maintained a pro-angiogenic and non-inflammatory status for at least 14 days. Here we explored, whether this aMO2 subset can positively influence the proliferation of human umbilical venous endothelial cells (HUVECs) without switching back into a pro-inflammatory (MO1) phenotype. aMO2 or HUVEC were seeded alone on glass cover slips (0.5 × 10(5) cells / 1.33 cm(2)) in a HUVEC specific cell culture medium (EGM-2) for 3 hrs, 24 hrs and 72 hrs or under co-culture conditions (0.5 × 10(5) HUVEC + 0.25 × 10(5) aMO2 / 1.33 cm(2)) in EGM-2 for the same time window as well (n = 6 each). Under co-culture conditions the numbers of adherent HUVEC per unit area were significantly higher (p < 0.01; 525 ± 52 HUVEC/mm(2)) compared to control mono-cultures (473 ± 76 HUVEC/mm(2)) after 72 hrs of cultivation and showed their typically spread morphology. The aMO2 remained in their subset status and secreted VEGF-A165 without release of pro-inflammatory cytokines until the end of the 72 hrs cultivation time period, thereby supporting the HUVEC proliferation. These in vitro results might indicate that this MO subset can be used as cellular delivery system for pro-angiogenic and non-inflammatory mediators to support the endothelialisation of biomaterials like e.g. cPnBA.
所谓的中间（MO2）单核细胞/巨噬细胞具有抗炎特性，并表达 MO 谱系标志物 CD163。在亲水丙烯酰胺基水凝胶上，经过血管生成刺激的人类中间（CD14++ CD16+）CD163++单核细胞/巨噬细胞（aMO2）至少保持促血管生成和非炎症状态 14 天。在这里，我们探讨了这种 aMO2 亚群是否可以在不转化为促炎（MO1）表型的情况下，正向影响人脐静脉内皮细胞（HUVEC）的增殖。aMO2 或 HUVEC 单独接种在玻璃载玻片上（0.5×10(5)个细胞/1.33 cm(2))，在 HUVEC 特异性细胞培养基（EGM-2）中培养 3 小时、24 小时和 72 小时，或在共培养条件下（0.5×10(5)个 HUVEC+0.25×10(5)个 aMO2/1.33 cm(2))在相同的时间窗口内培养（n=6 个/组）。在共培养条件下，与对照单核培养物（473±76 HUVEC/mm(2))相比，培养 72 小时后单位面积内黏附的 HUVEC 数量显著增加（p<0.01；525±52 HUVEC/mm(2))，并且表现出典型的铺展形态。aMO2 在 72 小时培养时间内保持其亚群状态，并分泌 VEGF-A165，而不释放促炎细胞因子，从而支持 HUVEC 的增殖。这些体外结果可能表明，这种 MO 亚群可用作细胞递送系统，用于输送促血管生成和非炎症性介质，以支持生物材料如 cPnBA 的内皮化。
