Institute of Food, Nutrition and Human Health, Massey University-Turitea, Tennent Drive, Palmerston North, New Zealand.
Br J Nutr. 2012 Sep 28;108(6):949-52. doi: 10.1017/S0007114511006490. Epub 2012 Jan 4.
Fe bioavailability can be manipulated by the nutritional composition of a meal. Ascorbic acid and unidentified components of meat, fish and poultry, but particularly beef, all appear to enhance the absorption of non-haem Fe. The aim of the present study is to identify whether extracts of green-lipped mussels (GLM; Perna canaliculus) enhance non-haem Fe absorption in Caco-2 cells and to compare the effect with that of beef. Raw GLM and raw beef homogenates were digested in vitro with pepsin at pH 2, and pancreatin and bile salts at pH 7. Tracer ⁵⁵Fe was used to measure cellular Fe uptake. Ascorbic acid was used as a positive control and egg albumin, exposed to the same in vitro digestion process, was used as a negative control. Caco-2 cell monolayers were incubated with treatments for 60 min. All values were standardised per μg of GLM, egg albumin, beef or ascorbic acid. The results showed that ascorbic acid enhanced non-haem Fe absorption to the highest degree. Beef and GLM digestates both significantly enhanced Fe absorption compared with egg albumin. In conclusion, GLM digestate significantly enhances non-haem Fe uptake in Caco-2 cells with a similar magnitude to that of beef.
膳食的营养成分可以影响铁的生物利用度。抗坏血酸和肉类、鱼类和禽类中未确定的成分，特别是牛肉，似乎都能增强非血红素铁的吸收。本研究旨在确定绿唇贻贝（GLM；Perna canaliculus）提取物是否能增强 Caco-2 细胞中非血红素铁的吸收，并将其与牛肉的效果进行比较。生 GLM 和生牛肉匀浆在 pH2 下用胃蛋白酶消化，在 pH7 下用胰蛋白酶和胆汁盐消化。用示踪 ⁵⁵Fe 测量细胞铁摄取量。抗坏血酸用作阳性对照，暴露于相同体外消化过程的卵白蛋白用作阴性对照。将 Caco-2 细胞单层与处理物孵育 60 分钟。所有值均按 GLM、卵白蛋白、牛肉或抗坏血酸的每微克进行标准化。结果表明，抗坏血酸能最大程度地促进非血红素铁的吸收。与卵白蛋白相比，牛肉和 GLM 消化物均能显著促进铁的吸收。综上所述，GLM 消化物能显著增强 Caco-2 细胞中非血红素铁的摄取，其效果与牛肉相当。
