Heart Research Center of Chonnam National University Hospital, Cardiovascular Research Institute of Chonnam National University, Gwangju 501-757, Korea.
Int J Toxicol. 2012 Jan-Feb;31(1):70-7. doi: 10.1177/1091581811423845. Epub 2012 Jan 3.
Sanguinarine is an alkaloid obtained from the bloodroot plant Sanguinaria canadensis and has beneficial effects on oxidative stress and inflammatory disorders. Previous reports have demonstrated that sanguinarine also exhibit anticancer properties. In the current study, we investigated the effects of sanguinarine on HT-29 human colon cancer cells. It was observed that sanguinarine treatment induces a dose-dependent increase in apoptosis of human colon cancer cells. We also investigated the effects of sanguinarine on the expression of apoptosis-associated proteins, and the results revealed that there was an increase in Bax and a decrease in B-cell lymphoma 2 (Bcl-2) protein levels. Moreover, sanguinarine treatment significantly increases the activation of caspases 3 and 9 that are the key executioners in apoptosis. Our results suggest that sanguinarine induces apoptosis of HT-29 human colon cancer cells and may have a potential therapeutic use in the treatment of human colon cancer.
血根碱是一种从血根植物中提取的生物碱，对氧化应激和炎症性疾病有有益的影响。先前的报告表明，血根碱也具有抗癌特性。在本研究中，我们研究了血根碱对 HT-29 人结肠癌细胞的影响。结果表明，血根碱处理诱导人结肠癌细胞凋亡呈剂量依赖性增加。我们还研究了血根碱对凋亡相关蛋白表达的影响，结果表明 Bax 蛋白水平增加，B 细胞淋巴瘤 2（Bcl-2）蛋白水平降低。此外，血根碱处理显著增加了凋亡的关键执行者 caspase 3 和 9 的激活。我们的结果表明，血根碱诱导 HT-29 人结肠癌细胞凋亡，可能在治疗人类结肠癌方面具有潜在的治疗用途。
