羊膜移植治疗角膜溃疡和持续性上皮缺损

Transplantation of amniotic membrane in corneal ulcers and persistant epithelial defects.

作者信息

Grzetić-Lenac Renata, Merlak Maja, Balog Tihomir, Babić Marijana Bilen, Dekaris Iva

机构信息

Department of Ophtalmology, Rijeka University Hospital Center, Rijeka, Croatia.

出版信息

Coll Antropol. 2011 Sep;35 Suppl 2:167-9.

PMID:22220427

Abstract

Amniotic membrane transplantation (AMT) leads to reduction of inflammatory symptoms and causes faster epithelisation in corneal ulcers and persistant epithelial defect. 21 patients with corneal ulcer (n = 18) or non-healing epithelial defect (n = 3) unresponsive to conventional treatment were included in the study. All patients were treated by AMT. Corneal epithelial cells in patients suffering from corneal ulcer secreted 3.51 +/- 1.79 of IL-1alpha, 64.27 +/- 31.53 pg/mL of TNFalpha and 209.07 +/- 201.82 pg/mL of VEGF. Levels of all 3 investigated cytokines were significantly higher as compared to controls (p < 0.005). Amniotic membranes that were used contained 775.69 +/- 613.98 pg/mL of IL-1alpha, 0.036 +/- 0.033 pg/mL of sTNF and 175.01 +/- 166.63 pg/mL of VEGF-R. Supporting effect of the AMT could be explained by the fact that AM secretes its natural antinflammatory antagonists IL-1ra, sTNF and VEGF-R.

摘要

羊膜移植（AMT）可减轻角膜溃疡和持续性上皮缺损的炎症症状，并使上皮化进程加快。本研究纳入了21例对传统治疗无反应的角膜溃疡患者（n = 18）或未愈合的上皮缺损患者（n = 3）。所有患者均接受了AMT治疗。角膜溃疡患者的角膜上皮细胞分泌白细胞介素-1α（IL-1α）3.51±1.79、肿瘤坏死因子-α（TNFα）64.27±31.53 pg/mL和血管内皮生长因子（VEGF）209.07±201.82 pg/mL。与对照组相比，所有3种研究细胞因子的水平均显著升高（p < 0.005）。所使用的羊膜含有IL-1α 775.69±613.98 pg/mL、可溶性肿瘤坏死因子（sTNF）0.036±0.033 pg/mL和血管内皮生长因子受体（VEGF-R）175.01±166.63 pg/mL。AMT的支持作用可以通过羊膜分泌其天然抗炎拮抗剂白细胞介素-1受体拮抗剂（IL-1ra）、sTNF和VEGF-R这一事实来解释。